Establishment and characterization of a porcine kidney cell line, FS-L3, which forms unique multicellular domes in serum-free culture

In Vitro Cellular & Developmental Biology. Animal
Y Sakoda, A Fukusho

Abstract

A stable porcine kidney epithelial cell line, FS-L3, was established and maintained in Eagle's minimum essential medium containing 0.295% tryptose phosphate broth, 0.5% Bacto Peptone, and 10 mM N, N-Bis (2-hydroxyethyl)-2-aminoethanesulfonic acid without any serum. The mode of chromosomes is 37 to 38. The FS-L3 cells formed fluid-filled, multicellular, three-dimensional domes on a single monolayer. The number of domes increased markedly after further cultivation. The origin of this cell line was confirmed as porcine by hybridization using PRE-1, which can be detected as a specific sequence in the porcine genome. It was also found that FS-L3 cells were free from possible adventitious viruses and mycoplasmas.

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Lever
Jan 1, 1992·Animal Genetics·H TakahashiH Yasue
Dec 1, 1991·Cell Structure and Function·H YasueP C Popescu
Mar 1, 1973·Archives of Biochemistry and Biophysics·K NoseH Katsuta
Jun 25, 1994·The Veterinary Record·S Edwards, J J Sands
Jul 1, 1994·Journal of Virological Methods·S R BolinR Roblin
Jan 1, 1987·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M A SmithJ V Bruckner

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Citations

Apr 28, 2000·Veterinary Microbiology·S EdwardsJ Westergaard
Oct 18, 2013·Journal of Virology·Junki MaruyamaAyato Takada
May 11, 2004·The Journal of Veterinary Medical Science·Yoshiko SekiguchiEiichi Honda
Dec 17, 2014·Journal of Microbiological Methods·Melissa ChapmanIan Macreadie
Nov 14, 2014·The Journal of Veterinary Medical Science·Tomokazu TamuraYoshihiro Sakoda
Apr 8, 2003·The Journal of Veterinary Medical Science·Ikuko SasakiEiichi Honda

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