Establishment and Characterization of a Stromal Cell Line Derived From a Patient With Thoracic Endometriosis

Reproductive Sciences
J GogusevP Validire

Abstract

Thoracic endometriosis (TE) syndrome is a clinical condition known as an extrapelvic form of endometriosis with the presence of functioning endometrial tissue involving lung parenchyma, pleura, chest wall, or diaphragm. In an effort to obtain an endometriosis ex vivo model, we established the spontaneously growing TH-EM1 cell line from endometriotic implants in lung parenchyma from a woman with TE. Maintained in long-term culture, the cells grew as large mesenchymal-like cells with a doubling time between 5 and 6 days. Treatment with medroxyprogesterone acetate (10-7 mol/L) inhibited the TH-EM1 cells growth and induced morphological changes to an epithelial-like cells. Strong expression of the nuclear estrogen receptors, progesterone receptors, and erytropoietin receptors were found in both the pulmonary implant and the TH-EM1 cells by immunohistochemical analysis. Consistent immunoreactivity of TH-EM1 cells for CD9, CD13, CD73, CD90, CD105, and CD157 was revealed by flow cytometry. Likewise, the embryonic markers, SRY-box 2 (SOX-2) and the Nanog molecules, were detected in 76% and 52% of the cells, while fetal hemoglobin and α-globin were detected in 76% and 65% of TH-EM1 cells, respectively. By RHG banding, normal metaphases ...Continue Reading

References

Feb 1, 1996·The American Journal of Medicine·J Joseph, S A Sahn
Aug 19, 1999·Clinical Obstetrics and Gynecology·G M Honoré
May 8, 2002·Molecular Human Reproduction·Rei YokomizoKunihiro Okamura
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Jan 15, 2003·Human Reproduction·Sachiko MatsuzakiKunihiro Okamura
May 20, 2004·AJR. American Journal of Roentgenology·Dipanka SarmaWilliam Halliday
Jul 9, 2004·JAMA : the Journal of the American Medical Association·Hugh S Taylor
Oct 1, 2004·The Journal of Thoracic and Cardiovascular Surgery·Stephan KoromWalter Weder
Mar 24, 2007·Experimental Hematology·Kevin J ZwezdarykAline B Scandurro
Mar 31, 2007·Annals of the New York Academy of Sciences·Hans-Jörg BühringWichard Vogel
Jul 12, 2007·Respiration; International Review of Thoracic Diseases·Areti AugouleaGeorge Christodoulakos
May 18, 2010·Current Opinion in Pulmonary Medicine·Marco Alifano
Jun 25, 2010·The New England Journal of Medicine·Linda C Giudice
Jul 20, 2010·Journal of the National Cancer Institute·Erika OrtolanAda Funaro
Sep 21, 2010·Fertility and Sterility·Martin GötteAndreas N Schüring
Jun 2, 2012·Frontiers in Bioscience (Elite Edition)·Tetsuo Maruyama, Yasunori Yoshimura
Oct 23, 2012·Journal of Minimally Invasive Gynecology·David SorianoMordechai Goldenberg
May 24, 2013·Annals of Thoracic and Cardiovascular Surgery : Official Journal of the Association of Thoracic and Cardiovascular Surgeons of Asia·Takahiro HagaHiroki Ebana
Nov 1, 2013·Nucleic Acids Research·Jeffrey R MacDonaldStephen W Scherer
Jun 4, 2015·Seminars in Reproductive Medicine·Serdar E BulunMatthew Dyson
Feb 13, 2018·Cytotherapy·Katarina Le Blanc, Lindsay C Davies

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