Establishment and characterization of a conditionally immortalized smooth muscle/myometrial-like cell line

Molecular Reproduction and Development
J QianJ L Lessard

Abstract

A novel smooth muscle/myometrial-like cell line, SMU1-10, has been generated from the uterus of a H-2Kb-tsA58 transgenic mouse carrying a thermolabile SV40 large T-antigen gene. These cells grow continuously when maintained at the permissive temperature (33 degrees C) for the SV40 large T-antigen but stop dividing when placed at the non-permissive temperature (39 degrees C) and ultimately die within 3 weeks. All of the SMU1-10 cells produce smooth muscle alpha-actin (SMAA) at both 33 degrees C and 39 degrees C. A subset of the cells also contain smooth muscle gamma-actin (SMGA), a hallmark of smooth muscle differentiation, and the fraction of cells staining for this actin increases from about 1% when maintained for three days at 33 degrees C to as much as 30% at 39 degrees C over the same length of time. However, the appearance of SMGA in SMU1-10 cells appears to be regulated mainly at a post-transcriptional level since in situ hybridization indicates that all cells contain SMGA mRNA at both 33 degrees C and 39 degrees C. SMU1-10 cultures also contain smooth muscle myosin heavy chain (SM-MHC) and SM22 alpha, both of which are only found in smooth muscle of the adult mouse. Three additional smooth muscle (myometrium)-related mar...Continue Reading

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Citations

Dec 25, 2003·American Journal of Respiratory and Critical Care Medicine·Limei ZhouMarc B Hershenson
Jul 24, 2002·Biology of Reproduction·Jennifer CondonWilliam E Rainey
Jun 28, 2017·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Marina IlicicJonathan W Paul
Mar 6, 2013·BMC Pregnancy and Childbirth·Andrea A MosherDonna M Slater
May 26, 2017·BioMed Research International·Marina IlicicJonathan W Paul

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