Establishment and characterization of an embryonic pericyte cell line

Microcirculation : the Official Journal of the Microcirculatory Society, Inc
Huaning ZhaoJohn C Chappell

Abstract

Pericytes are specialized perivascular cells embedded within the basement membrane. These cells envelope the abluminal surface of endothelial cells and promote microvessel homeostasis. Recent discoveries of unique pericyte functions, particularly in neural tissues, underscore the need for overcoming existing challenges in establishing a functionally validated pericyte cell line. Here, we present methodologies for addressing these challenges as well as an embryonic pericyte cell line for use with in vitro and ex vivo experimental models. We isolated an enriched population of NG2:DsRed+ pericytes from E12.5 mice. This pericyte cell line was compared to MEFs with respect to gene expression, cell morphology and migration, and engagement with endothelial cells during junction stabilization and angiogenesis. NG2+ pericytes displayed gene expression patterns, cell morphology, and 2D migration behaviors distinct from MEFs. In three different vessel formation models, pericytes from this line migrated to and incorporated into developing vessels. When co-cultured with HUVECs, these pericytes stimulated more robust VE-Cadherin junctions between HUVECs as compared to MEFs, as well as contributed to HUVEC organization into primitive vascular...Continue Reading

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Citations

May 9, 2019·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Laura B PayneJohn C Chappell
May 29, 2020·Journal of Cellular and Molecular Medicine·Han SuJian-Xiong Chen
Apr 16, 2019·Journal of Biological Engineering·Huaning Zhao, John C Chappell
Mar 24, 2021·Journal of Cell Science·Claire A DessallesAbdul I Barakat
Mar 23, 2021·Current Tissue Microenvironment Reports·Laura Beth PayneJohn C Chappell
Jun 15, 2021·Frontiers in Cell and Developmental Biology·Georgina GossFiona M Watt

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