Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids

Virology
Z Q YuanL Nasir

Abstract

It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of primary fibroblasts with BPV-1 DNA. Previously detected BPV-1 genome variations in equine sarcoids are also found in sarcoid cell lines, and only variant BPV-1 genomes can transform equine cells. These equine cell lines are morphologically transformed, proliferate faster than parental cells, have an extended life span and can grow independently of substrate. These characteristics are more marked the higher the level of viral E5, E6 and E7 gene expression. These findings confirm that the virus has an active role in the induction of sarcoids and the lines will be invaluable for further studies on the role of BPV-1 in sarcoid pathology.

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Citations

Aug 13, 2013·BioMed Research International·Gennaro AltamuraGiuseppe Borzacchiello
Dec 3, 2014·The Veterinary Journal·S MosseriA Kipar
Sep 13, 2013·BMC Veterinary Research·Tina RichJanet C Patterson-Kane
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Jan 20, 2021·Veterinary Pathology·Magdalena OgłuszkaAndrzej Raś
Mar 20, 2012·BMC Veterinary Research·Maria StrazzulloGiuseppe Borzacchiello

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