Establishment and characterization of four malignant pleural mesothelioma cell lines from Japanese patients

Cancer Science
Noriyasu UsamiToyoaki Hida

Abstract

Malignant pleural mesothelioma (MPM) is an asbestos-related malignancy that is highly resistant to current therapeutic modalities. We established four MPM cell lines (ACC-MESO-1, ACC-MESO-4, Y-MESO-8A and Y-MESO-8D) from Japanese patients, with the latter two from the same patient with biphasic-like characteristics of MPM, showing epithelial and sarcomatous phenotypes, respectively, in cell culture. These cells grew well in RPMI-1640 medium supplemented with 10% fetal bovine serum under 5% CO2. Mutation and expression analyses demonstrated that the tumor suppressor gene NF2, which is known to be one of the most frequently mutated in MPM, is mutated in ACC-MESO-1. We detected homozygous deletion of p16INK4A/p14ARF in all four MPM cell lines. However, mutations of other tumor suppressor genes, including TP53, and protooncogenes, including KRAS, NRAS, BRAF, EGFR and HER2, were not found in these cell lines. Polymerase chain reaction amplification of the simian virus 40 sequence did not detect any products. We also analyzed genetic alterations of six other MPM cell lines and confirmed frequent mutations of NF2 and p16INK4A/p14ARF. To characterize the biological differences between Y-MESO-8A and Y-MESO-8D, we carried out cDNA microa...Continue Reading

References

Jan 21, 1991·International Journal of Cancer. Journal International Du Cancer·L S ManningB W Robinson
Jul 1, 1990·Cancer Genetics and Cytogenetics·A HagemeijerH C Hoogsteden
Aug 15, 1989·International Journal of Cancer. Journal International Du Cancer·M A VersnelA Hagemeijer
Apr 1, 1995·The Annals of Thoracic Surgery·H I PassW J Matthews
Jan 1, 1996·Journal of Cellular Biochemistry. Supplement·R M PhelpsJ L Mulshine
Jan 23, 1998·Chest·D J Sugarbaker, J J Norberto
Jun 3, 2000·Japanese Journal of Cancer Research : Gann·M SatoY Hasegawa
Oct 16, 2002·Cancer Genetics and Cytogenetics·Yoshitaka SekidoKaoru Shimokata
Dec 3, 2002·Nature Reviews. Cancer·Adi F GazdarMichele Carbone
Nov 6, 2003·International Journal of Cancer. Journal International Du Cancer·Mika UchiyamaYoshitaka Sekido
Jun 16, 2004·Current Problems in Cancer·Harvey I PassMichele Carbone
Nov 24, 2004·Thoracic Surgery Clinics·Harvey I PassMichele Carbone
Apr 5, 2005·Cancer Research·James J ManfrediStuart A Aaronson
Aug 2, 2005·Lancet·Bruce W S RobinsonRichard A Lake
Oct 14, 2005·The New England Journal of Medicine·Bruce W S Robinson, Richard A Lake

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Citations

Mar 1, 2008·Environmental Health and Preventive Medicine·Yoshitaka Sekido
Aug 14, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Takeshi HanagiriFumihiro Tanaka
Jul 29, 2010·The Journal of Antibiotics·Keiichiro MotohashiKazuo Shin-ya
Aug 4, 2011·The Journal of Antibiotics·Miho IzumikawaKazuo Shin-Ya
Feb 18, 2011·The Journal of Antibiotics·Jun-ya UedaKazuo Shin-ya
Nov 3, 2011·The Journal of Antibiotics·Miho IzumikawaKazuo Shin-ya
Feb 26, 2013·Cancer Cell International·Motohiko SatohShunichiro Kubota
Nov 17, 2011·Annals of Surgical Oncology·Mihoko HorioYoshinori Hasegawa
Sep 8, 2009·Bioscience, Biotechnology, and Biochemistry·Miho IzumikawaKazuo Shin-Ya
Oct 11, 2011·PloS One·Mutsumi Kanamori-KatayamaYoshihide Hayashizaki
Mar 22, 2013·PloS One·Vandana RelanRayleen V Bowman
Nov 25, 2011·International Journal of Nanomedicine·Hisao HaniuNaoto Saito
Jan 10, 2012·International Journal of Nanomedicine·Hisao HaniuHiroyuki Kato
Jan 24, 2012·International Journal of Nanomedicine·Hisao HaniuHiroyuki Kato
May 3, 2014·International Journal of Nanomedicine·Hisao HaniuHiroyuki Kato
Aug 8, 2009·Bioscience, Biotechnology, and Biochemistry·Keiichiro MotohashiKazuo Shin-ya
Feb 20, 2016·Cell Death and Differentiation·T ChernovaM MacFarlane
Jun 23, 2012·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Futoshi IshiguroYoshitaka Sekido
Sep 7, 2007·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Manish R PatelRobert A Kratzke
Feb 28, 2009·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Steven G GrayKenneth J O'Byrne
May 31, 2011·Trends in Pharmacological Sciences·Roberto E Favoni, Tullio Florio
Feb 23, 2010·Biochemical and Biophysical Research Communications·Yukitoshi TakemuraShunichiro Kubota
Aug 30, 2008·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Sumitaka HasegawaTsuneo Saga
Apr 9, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Naruyuki KobayashiHiroshi Date
Sep 9, 2008·Cancer Science·Li JiangShinya Toyokuni
Apr 19, 2012·International Journal of Cancer. Journal International Du Cancer·Momen ElshazleyYoshinori Hasegawa
Jan 28, 2010·International Journal of Cancer. Journal International Du Cancer·Yoshinori SuzukiKunio Matsumoto
Jan 27, 2010·Cancer Science·Koichi InamiOkio Hino
Jun 20, 2015·BioMed Research International·Kayo MaruyamaHiroyuki Kato
May 17, 2015·Cancer Treatment Reviews·V PanouO D Røe

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