Abstract
There is a need to establish animal models which are suitable for investigation of human gastric cancer metastasis to the liver. To this end, a human gastric carcinoma line, AZ521 was injected into the spleens of nude mice. Cells from the few liver metastatic foci of injected AZ521 were expanded "in vitro" and subsequently injected into the spleens of nude mice. By repeating these procedures three times, we were able to obtain a cell line, designated as AZ-H3c, with high metastatic potential in nude mice. Liver metastasis developed in 15 of 21 (71%) animals injected with AZ-H3c, but only in 14% of those injected with parental AZ521. Further, AZ-H3c caused faster tumor development than did AZ521. However, the primary AZ-H3c tumors and liver metastatic AZ-H3c tumors showed essentially the same histological appearance. We also analyzed the cell surface expression of adhesion molecules. The data showed that the expression of VLA-1, VLA-2, VLA-3, VLA-4, VLA-5 was enhanced in AZ-H3c. In contrast, the expression of VLA-6, (alpha(v)beta3), E-cadherin, ICAM-1 and LFA-1 was reduced in this high-metastatic line. These results suggest that (beta1) integrins play an important role in the liver metastasis of human gastric carcinoma cells. Ou...Continue Reading
References
Dec 1, 1992·Japanese Journal of Cancer Research : Gann·S KawaguchiT Uede
Jun 13, 1992·Lancet·I R Hart, A Saini
Jul 30, 1992·International Journal of Cancer. Journal International Du Cancer·X WangR M Hoffman
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·X Y FuR M Hoffman
Aug 2, 1990·Nature·T A Springer
May 17, 1991·Cell·W G CarterP J Gahr
Dec 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Dedhar
Mar 9, 1990·Cell·F G Giancotti, E Ruoslahti
Apr 1, 1990·British Journal of Cancer·M PignatelliW F Bodmer
Oct 1, 1990·Japanese Journal of Cancer Research : Gann·Y IkedaA Kono
Jan 1, 1990·Annual Review of Immunology·M E Hemler
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M J Elices, M E Hemler
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Apr 1, 1988·Immunology Today·M E Hemler
Dec 1, 1988·Nature·A SonnenbergF Hogervorst
Feb 27, 1987·Cell·R O Hynes
Jan 1, 1985·Cell·R PytelaE Ruoslahti
Jul 25, 1986·Science·M J HumphriesK M Yamada
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·Y FradetL J Old
May 15, 1980·International Journal of Cancer. Journal International Du Cancer·R T MorganS G Gordon
Jan 1, 1995·Surgery·G P GuiR Carpenter
Feb 1, 1995·Circulation Research·R T LeeM E Hemler
Oct 1, 1994·Cell Adhesion and Communication·J M BergelsonR W Finberg
Oct 15, 1993·Cancer Research·T KageshitaS Ferrone
May 6, 1994·Cell·F QianI L Weissman
Oct 1, 1993·Current Opinion in Cell Biology·M Takeichi
Oct 1, 1993·Current Opinion in Cell Biology·R L Juliano, J A Varner
May 1, 1993·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S Kawaguchi, T Uede
Citations
Jun 29, 1999·Surgery Today·T ShishidoN Sato
Jan 30, 2007·Clinical & Experimental Metastasis·Xin-Zhong ChangZhi-Min Shao
Jan 21, 2012·Clinical & Experimental Metastasis·Rosa BoschRamon Mangues
Aug 11, 2000·Cancer Letters·H FuruyamaM Imamura
Oct 12, 2010·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M ScartozziS Cascinu
May 30, 2013·Haematologica·Rosa BoschRamon Mangues
Nov 22, 2001·Biochemical and Biophysical Research Communications·H IjichiM Omata
Feb 4, 2014·Therapeutic Delivery·Kazuyoshi YanagiharaYasuhiro Matsumura
Aug 2, 2005·The Journal of Surgical Research·R FukuiK Hirata
Sep 12, 1998·The Journal of Surgical Research·K OkazakiH Itoh
Dec 9, 2009·Molecular Oncology·Kristin SnipstadRuth H Paulssen
Jul 28, 2001·Japanese Journal of Cancer Research : Gann·H NomuraK Hirata
Dec 4, 2002·Japanese Journal of Cancer Research : Gann·Tsuneo IkenoueMasao Omata
Sep 1, 1996·Japanese Journal of Cancer Research : Gann·T ShishidoK Hirata
Aug 2, 2000·Japanese Journal of Cancer Research : Gann·H NishimoriK Hirata
Jul 9, 2004·Cells, Tissues, Organs·Rong-Yaun ShyuTzu-Ming Chang
Apr 21, 2009·Cancer Letters·Wei-Dong ShiLu-Ming Liu
Mar 9, 2017·Scientific Reports·Yusuke KandaFutoshi Okada
May 10, 2001·Pancreas·H NishimoriK Hirata
Oct 6, 1997·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·X LiF Shinozaki
Jun 6, 2007·Human Cell·Satoshi OhiHiroshi Ishikawa
Jul 13, 2004·Cancer Science·Kazuyoshi YanagiharaSetsuo Hirohashi
Feb 25, 1998·Japanese Journal of Cancer Research : Gann·T SagaJ Konishi
Mar 15, 2002·Pancreas·Hidefumi NishimoriKoichi Hirata
Sep 22, 2006·American Journal of Physiology. Cell Physiology·Yong-Bae KimJung Weon Lee
Mar 7, 2020·Aging·Lijing WangXiangdong Cheng
May 31, 2002·Oncogene·Tae Hyeon KooJeong-Hyung Lee
Jul 11, 2003·World Journal of Gastroenterology : WJG·Ji-Cheng ZhangXiao-Min Wang
Mar 6, 2007·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·M BasogluD Oren
Jan 31, 2002·World Journal of Gastroenterology : WJG·Y LiW H Bao
Jan 31, 2002·World Journal of Gastroenterology : WJG·Jing-Jing SunYin-Kun Liu
Mar 8, 2017·International Journal of Molecular Sciences·Xiaoqin LiuJinjun Li
Dec 6, 2006·Cancer Research·Tsuyoshi EnomotoNobuhiro Ohkohchi
May 16, 2003·Pathology, Research and Practice·Atsuhiro KawashimaYoshio Oda
Jan 24, 2013·Journal of Medicinal Chemistry·Eider San SebastiánFrancisco J Blanco