Establishment and characterization of patient-derived xenograft and its cell line of primary leiomyosarcoma of bone

In Vitro Cellular & Developmental Biology. Animal
Rieko OyamaTadashi Kondo

Abstract

Primary leiomyosarcoma (LMS) of bone is a rare and aggressive mesenchymal malignancy that differentiates toward smooth muscle. Complete resection is the only curable treatment, and novel therapeutic approaches for primary LMS of bone have long been desired. Patient-derived xenografts (PDXs) and cell lines are invaluable tools for preclinical studies. Here, we established PDXs from a patient with primary LMS of bone and a cell line from an established PDX. Bone primary LMS tissue was subcutaneously implanted into highly immune-deficient mice. After two passages, a piece of the tumor was subjected to tissue culturing, and a morphological evaluation and proteomic analysis were performed on the PDX and the established cell line. Moreover, the responses of the established cell line to anti-cancer drugs were examined. Microscopic observations revealed that the PDX tumors retained their original histology. The cell line was established from the third-generation PDX and named NCC-LMS1-X3-C1. The cells were maintained for over 18 mo and 40 passages. The cells exhibited a spindle shape and aggressive growth. Mass spectrometric protein identification revealed that the original tumor tissue, PDX tumor tissue, and NCC-LMS1-X3-C1 cells had s...Continue Reading

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Citations

Apr 23, 2020·Sarcoma·Kirill I KirsanovMarianna G Yakubovskaya
Oct 17, 2018·In Vitro Cellular & Developmental Biology. Animal·Rieko OyamaTadashi Kondo
Oct 30, 2020·Cancers·Emanuela PalmeriniEric L Staals

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Methods Mentioned

BETA
surgical resection
xenografts
xenograft
PCR

Software Mentioned

MASCOT
PeakScanner
R package “ treemap ”

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