Establishment and characterization of six human gastric carcinoma cell lines, including one naturally infected with Epstein-Barr virus.

Cellular Oncology (Dordrecht)
Ja-Lok KuJae-Gahb Park

Abstract

We report the characterization of six new gastric carcinoma cell lines (designated NCC-19, NCC-20, NCC-24, NCC-59, SNU-1750 and SNU-1967) established from primary tumor samples of Korean patients. Four cell lines grew as adherent monolayers, one as both adherent and floating cell clumps and one as floating cell aggregates. The cell phenotypes, including the histopathology of the primary tumors and in vitro growth characteristics, were determined. We also performed molecular characterization, including DNA fingerprinting analysis and abnormalities of K-ras, p53, β-catenin, and TGF-βRII genes by PCR-SSCP and sequencing analyses. Population doubling times varied from 47-135 h. All cell lines showed relatively high viability, absence of mycoplasma or bacteria contamination and genetic heterogeneity by DNA fingerprinting analysis. Three lines had p53 mutations; one line had mutations in codon 13 (Gly13Asp) in K-ras and no line had a β-catenin mutation. NCC-59 cell line had a -1-bp mutation in 10-bp poly deoxy adenine repeat tract of the TGF-βRII gene. Moreover, NCC-24 gastric cancer cell line was found to be infected with Epstein-Barr virus (EBV). EBV infection was also shown in the original carcinoma tissue of the NCC-24 cell line....Continue Reading

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Citations

Nov 12, 2014·Cancers·Jun NishikawaIsao Sakaida
Jul 25, 2015·International Journal of Cancer. Journal International Du Cancer·Kwai Fung HuiAlan Kwok Shing Chiang
Sep 29, 2018·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Sho SasakiIsao Sakaida
Sep 19, 2020·Cancer Cell International·Jigui PengJianchun Cai
Feb 26, 2019·Genomics·Yu-Long BaiYao-Zhong Liu

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