Establishment and Characterization of the Novel High-Grade Serous Ovarian Cancer Cell Line OVPA8

International Journal of Molecular Sciences
Patrycja TudrejKatarzyna M Lisowska

Abstract

High-grade serous ovarian carcinoma (HGSOC) is the most frequent histological type of ovarian cancer and the one with worst prognosis. Unfortunately, the majority of established ovarian cancer cell lines which are used in the research have unclear histological origin and probably do not represent HGSOC. Thus, new and reliable models of HGSOC are needed. Ascitic fluid from a patient with recurrent HGSOC was used to establish a stable cancer cell line. Cells were characterized by cytogenetic karyotyping and short tandem repeat (STR) profiling. New generation sequencing was applied to test for hot-spot mutations in 50 cancer-associated genes and fluorescence in situ hybridization (FISH) analysis was used to check for TP53 status. Cells were analyzed for expression of several marker genes/proteins by reverse-transcription polymerase chain reaction (RT-PCR), fluorescence-activated cell sorting (FACS), and immunocytochemistry (ICC). Functional tests were performed to compare OVPA8 cells with five commercially available and frequently used ovarian cancer cell lines: SKOV3, A2780, OVCAR3, ES2, and OAW42. Our newly-established OVPA8 cell line shows morphologic and genetic features consistent with HGSOC, such as epithelial morphology, mu...Continue Reading

References

Feb 1, 1977·Journal of the National Cancer Institute·J FoghJ D Loveless
Oct 2, 2002·Molecular Pathology : MP·G EllisonJ C Fox
Nov 27, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tanya J ShawBarbara C Vanderhyden
Sep 28, 2006·Breast Cancer Research and Treatment·James M RaeMichael D Johnson
Nov 8, 2006·Molecular Cancer Therapeutics·Ogechi N IkediobiRichard Wooster
Aug 30, 2008·The American Journal of Surgical Pathology·Daisuke NonakaRobert A Soslow
Jun 25, 2009·Cancer Research·Ann F Chambers
Feb 16, 2010·The American Journal of Surgical Pathology·Robert J Kurman, Ie-Ming Shih
May 11, 2010·Journal of Biomedicine & Biotechnology·Cord C UphoffHans G Drexler
Apr 20, 2011·Journal of Applied Genetics·Izabela BrozekJanusz Limon
Dec 7, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Adam D StegCharles N Landen
Aug 29, 2012·International Journal of Clinical Oncology·Hiroaki Itamochi, Junzo Kigawa
Jul 11, 2013·Nature Communications·Silvia DomckeNikolaus Schultz
Feb 12, 2014·Journal of Visualized Experiments : JoVE·Bradley HoweFern Tsien
Jun 18, 2015·Nature Communications·Tan A InceGordon B Mills
Sep 1, 2015·Gynecologic Oncology·Kevin M EliasRonny Drapkin
Oct 21, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Karina HewFiona Simpkins
Dec 17, 2015·Postȩpy higieny i medycyny doświadczalnej·Katarzyna Aleksandra Kujawa, Katarzyna Marta Lisowska
Mar 31, 2016·Journal of Cancer Research and Clinical Oncology·Katarzyna M LisowskaJolanta Kupryjańczyk
Jan 1, 2015·Gynecologic Oncology Research and Practice·Lauren Patterson CobbAngeles Alvarez Secord
Mar 23, 2017·Molecular Cancer·Michela Lupia, Ugo Cavallaro
May 13, 2017·Advances in Medical Sciences·Małgorzata KrześniakMarek Rusin
Dec 19, 2017·Cancer Chemotherapy and Pharmacology·Alexander J CortezKatarzyna M Lisowska
Apr 10, 2018·Contemporary Oncology Współczesna Onkologia·Aleksandra KlembaClaudine Kieda

❮ Previous
Next ❯

Citations

Sep 22, 2019·Diagnostics·Patrycja TudrejKatarzyna Marta Lisowska
Sep 24, 2020·Cancers·Alexander Jorge CortezKatarzyna Marta Lisowska
Oct 27, 2020·Frontiers in Oncology·Anja Charlotte Lundgren MortensenDiana Spiegelberg
Dec 4, 2020·International Journal of Molecular Sciences·Agnieszka SzyposzynskaAleksandra Klimczak
Apr 27, 2021·Frontiers in Oncology·Kamila KitowskaRafal Sadej
May 1, 2021·International Journal of Molecular Sciences·Paweł UruskiKrzysztof Książek
Jun 3, 2021·International Journal of Environmental Research and Public Health·Sheril June AnkashaNorfilza Mohd Mokhtar
Jan 8, 2022·Oncology Letters·Nadeeya Mad-AdamPotchanapond Graidist

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
xenografts
electrophoresis
flow cytometry
Chip
Reverse-Transcription PCR

Software Mentioned

GeneTools
AxioVision Microscope
GraphPad Prism
ANNOVAR
Ion Reporter
GeneMapper
STATISTICA
Ikaros

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Breast Cancer: BRCA1 & BRCA2

Mutations involving BRCA1, found on chromosome 17, and BRCA2, found on chromosome 13, increase the risk for specific cancers, such as breast cancer. Discover the last research on breast cancer BRCA1 and BRCA2 here.