Establishment and characterization of two cell lines derived from primary cultures of Gekko japonicus cerebral cortex

Cell Biology International
Mei LiuXiaosong Gu

Abstract

Adult Gekko japonicus is one of those vertebrates that are able to regenerate their missing or amputated tail. The most interesting feature of this animal lies in the ability of its spinal cord to regrow a functional tail. A fundamental question is whether the neuroglial cells play a different role compared with high vertebrates. Since in vitro studies using primary neuroglial cells are hampered by the limited lifespan and miscellaneous genetic background of these cells, we generated neuroglial cell lines from primary cell cultures of cerebral cortex of G. japonicus. The SV40 (simian-virus-40) T antigen gene was introduced into primary cell cultures. Cell cycle analysis, cell growth and proliferation, cell colony formation and contact inhibition, as well as karyotype assays were investigated. Two cell colonies, Gsn-1 and Gsn-3, were immunochemically characterized as glial fibrillary acidic protein and galactocerebroside-positive respectively. Compared with parental primary cells, the Gsn cells displayed shorter population doubling time, decreased percentage of cells in the G0/G1 phase, higher cell proliferation index, and increased cell activity. In assays of colony characteristics, Gsn cells showed increased cell activity at t...Continue Reading

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Citations

Sep 27, 2012·The International Journal of Biochemistry & Cell Biology·Yingjie WangYongjun Wang
Sep 3, 2014·Journal of Molecular Neuroscience : MN·Honghua SongYongjun Wang
Jan 29, 2020·Andrologia·Sunny O AbarikwuShio Kumar Singh

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