PMID: 11933048Apr 5, 2002Paper

Establishment and characterization of immortalized neuronal cell lines derived from the spinal cord of normal and trisomy 16 fetal mice, an animal model of Down syndrome

Journal of Neuroscience Research
Ana María CárdenasPablo Caviedes

Abstract

We report the establishment of continuously growing cell lines from spinal cords of normal and trisomy 16 fetal mice. We show that both cell lines, named M4b (derived from a normal animal) and MTh (trisomic) possess neurological markers by immunohistochemistry (neuron specific enolase, synaptophysin, microtubule associated protein-2 [MAP-2], and choline acetyltransferase) and lack glial traits (glial fibrillary acidic protein and S100). MTh cells were shown to overexpress mRNA of Cu/Zn superoxide dismutase, whose gene is present in autosome 16. We also studied intracellular Ca2+ signals ([Ca2+]i) induced by different agonists in Indo-1 loaded cells. Basal [Ca2+]i was significantly higher in MTh cells compared to M4b cells. Glutamate (200 microM) and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACDP) (100 microM) induced rapid, transient increases in [Ca2+]i in M4b and MTh cells, indicating the presence of glutamatergic metabotropic receptors. N-methyl-D-aspartate (NMDA) and kainate, but not alpha-amino-hydroxy-5-methylisoxazole-4-propionic acid (AMPA), produced [Ca2+)]i rises in both cell types. MTh cells exhibited faster time-dependent decay phase kinetics in glutamate-induced responses compared to M4b cells. Nicotine in...Continue Reading

References

Sep 1, 1992·Neurobiology of Aging·M P Mattson, R E Rydel
Sep 1, 1988·Trends in Neurosciences·J T CoyleJ D Gearhart
Jan 1, 1985·Annals of the New York Academy of Sciences·C J EpsteinL B Epstein
Jan 1, 1983·Progress in Neurobiology·B S ScottT L Petit
Oct 1, 1995·Nature Genetics·R H ReevesM T Davisson
Jul 1, 1993·Journal of Molecular and Cellular Cardiology·P CaviedesE Jaimovich
Jun 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C Q Lin, M J Bissell
Apr 1, 1993·Brain Research. Molecular Brain Research·M KengakuT Deguchi
Nov 14, 1997·Journal of Muscle Research and Cell Motility·J L LiberonaE Jaimovich
May 13, 1999·Neuroreport·C Martínez-CuéJ Flórez
Oct 6, 1999·Current Opinion in Cell Biology·C Streuli
Sep 21, 2000·The European Journal of Neuroscience·D D AllenP Caviedes
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·D A Sinclair

❮ Previous
Next ❯

Citations

Oct 14, 2005·Drug Development and Industrial Pharmacy·David D AllenPablo A Caviedes
Jan 15, 2003·Journal of Neuroscience Research·Carmen MontielAna M Cárdenas
May 2, 2006·Journal of Physiology, Paris·Katherine SaudPablo Caviedes
Sep 23, 2014·Frontiers in Cellular Neuroscience·Fanny MomboisseAna M Cárdenas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.