PMID: 6982237Aug 15, 1982Paper

Establishment and cytological characteristics of two in vitro T-cell lines derived from a child with acute lymphatic leukemia and a man with adult T-cell leukemia in Japan

International Journal of Cancer. Journal International Du Cancer
M NagasakaT Sugiyama

Abstract

Two permanent T-cell leukemia lines designated KH-1 and KH-2 were established from the peripheral blood of a 9-year-old boy with acute lymphoblastic leukemia and a 47-year-old man with adult T-cell leukemia (ATL). No T-cell growth factor was used. KH-1 cells grew as single cells and KH-2 cells formed clusters in suspension culture. Erosette formation, the absence of immunoglobulin determinants and Epstein-Barr-virus-associated nuclear antigen, and the presence of T-cell antigens revealed by monoclonal antibodies were characteristics of these cell lines as in other established T-cell leukemia lines. Chromosome analysis at the beginning revealed mosaic presence of cells with 46, XY, t(8q+; 15q-) and 46, XY which was later completely replaced by the latter karyotype in KH-1, and abnormal karyotype, 47, XY, +3, t (8q-; 10p+) was maintained throughout the period of in vitro passage in KH-2. The donor patient of KH-2 formerly lived in the south-western part of Japan where ATL is considered endemic and numerous type-C virus particles were detected electron microscopically, in KH-2 cell pellets.

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Citations

Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H HoshinoT Sugimura
Sep 1, 1990·Journal of Clinical Pathology·Y TokudaT Sugiyama
Oct 15, 1989·International Journal of Cancer. Journal International Du Cancer·T KoizumiT Fujita
Jul 15, 1983·International Journal of Cancer. Journal International Du Cancer·F A Vyth-Dreese, J E de Vries
Feb 15, 1984·International Journal of Cancer. Journal International Du Cancer·T AokiR C Gallo
Aug 15, 1986·International Journal of Cancer. Journal International Du Cancer·T KatohT Wakutani
Apr 10, 2002·Pathology International·Riko KitazawaSakan Maeda
Mar 1, 1990·Journal of Clinical Pathology·U LatzaH Stein
Oct 16, 2007·The Journal of Pathology·S TedoldiT Marafioti
Mar 1, 1986·Acta Pathologica Japonica·J HaratakeH Sato
Jan 1, 1992·Microbiology and Immunology·T NakagamiK Yamanishi
Sep 14, 1992·Biochimica Et Biophysica Acta·J Sodroski

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