Establishment and evaluation of a stable steroidogenic caprine luteal cell line

Theriogenology
Wei LiDewen Tong

Abstract

Many physiological, biological, pharmacologic, and toxicologic events and compounds affect the function of Saanen dairy goat luteal cells, resulting in implantation failure or early embryonic loss. Although primary luteal cell cultures have been used, their finite lifespan precludes assessment of long-term effects. In the present study, primary caprine luteal cells (CLCs) were immortalized through transfection of a plasmid containing the human telomerase reverse transcriptase (hTERT) gene. The expression of hTERT and telomerase activity were evaluated in transduced CLCs (hTERT-CLCs). In this study, these cells steadily expressed hTERT gene and exhibited higher telomerase activity at Passages 30 and 50. The hTERT-CLCs at Passages 30 and 50 expressed genes encoding key proteins, enzymes and receptors inherent to normal luteal cells, e.g., steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc), 3β-hydroxysteroid dehydrogenase (3β-HSD), and LH-receptor (LH-R). In addition, immortalized caprine luteal cells produced detectable quantities of progesterone in response to 8-bromo-cAMP (8-Br-cAMP) or 22(R)-hydroxycholesterol (22R-HC) stimulation. Furthermore, this cell line appeare...Continue Reading

References

Sep 1, 1978·Steroids·G O PottsH P Schane
Jun 20, 1992·Journal of Molecular Biology·M Z LevyC B Harley
Jan 1, 1987·Reproductive Toxicology·M C Rao, G Gibori
Nov 1, 1993·In Vitro Cellular & Developmental Biology. Animal·T W BriersH Vanderstichele
Jan 28, 1999·Biochemical and Biophysical Research Communications·M M OuelletteJ W Shay
Jun 5, 2002·Molecular and Cellular Endocrinology·F J DiazM C Wiltbank
Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Isabelle GuilleretJean Benhattar
Feb 21, 2004·Biochemical and Biophysical Research Communications·Yin XiaoxueWang Shenwu
May 1, 1996·Theriogenology·R A MilvaeJ C Carlson
Nov 2, 2006·Endocrine Reviews·Carlos StoccoGeula Gibori
Jan 30, 2007·Prostaglandins & Other Lipid Mediators·D P Mishra, A Dhali
Mar 24, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Ewa BorowczykAnna T Grazul-Bilska
Nov 13, 2008·Cytotechnology·Kwang M LeeMichel M Ouellette
Mar 10, 2009·Cell Biology International·Yasuo Inoshima, Naotaka Ishiguro
Nov 28, 2009·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Shahid Afzal GillAhrar Khan
Jul 3, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Nae-Fang TwuChih-Hsien Chiu

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Citations

Jul 1, 2014·Journal of Biochemical and Molecular Toxicology·Wei LiDewen Tong
Oct 16, 2015·Animal Science Journal = Nihon Chikusan Gakkaihō·Jinhua ZhouYaping Jin
Mar 5, 2016·Cell Biology International·Jiajia XiaoYong Zhang
Feb 2, 2013·Developmental Cell·Yuliya Sytnikova, Nelson C Lau
Oct 31, 2016·Environmental Toxicology and Pharmacology·Chenchen WuBaoyu Zhao

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