Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses

PloS One
Wenxiu WangZhiqiang Shen

Abstract

In this study, we report the establishment and characterization of a new epithelial cell line, goose embryonated epithelial cell line (GEE), derived from embryonic goose tissue. The purified GEE cell line can efficiently grow over 65 passages in the M199 medium supplemented with 10% fetal bovine serum at 37°C. Immunofluorescence assay was used to identify purified GEE cells as epithelial cell line by detecting expression of the Keratin-18 and -19. Further characterizations demonstrated that the GEE cell line can be continuously subcultured with (i) a high capacity to replicate for over 65 passages, (ii) a spontaneous epithelial-like morphology, (iii) constant chromosomal features and (iv) without an evidence of converting to tumorigenic cells either in vitro or in vivo study. Moreover, the GEE cell line can be effectively transfected with plasmids expressing reporter genes of different avian viruses, such as VP3, VP1 and F of goose parvo virus (GPV), duck hepatitis virus (DHV), and Newcastle disease virus (NDV), respectively. Finally, the established GEE cell line was evaluated for avian viruses infection susceptibility. Our results showed that the tested GPV, DHAV and NDV were capable to replicate in the new cell line with tit...Continue Reading

References

Jul 1, 1977·Experimental Cell Research·H Beug, T Graf
Oct 1, 1988·Trends in Genetics : TIG·E Fuchs
May 1, 1988·Cell and Tissue Research·W JelkmannF P Seydel
Mar 15, 1974·Experimental Cell Research·G O GeyF B Bang
Jan 1, 1969·The Journal of Heredity·S E Bloom
Sep 1, 1965·Experimental Cell Research·H SchneiderA Smith
May 1, 1982·Cytometry·R ZippelL Alberghina
Oct 15, 1998·The Veterinary Record·D J AlexanderG Parsons
Dec 18, 2003·Hybridoma and Hybridomics·Marina B MelixetianTatyana M Grinchuk
Aug 13, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kathy K H SvobodaMarion K Gordon
Jun 30, 2010·Molecular Biotechnology·Seiichi YamanoAmr M Moursi
Jan 19, 2012·Human Vaccines & Immunotherapeutics·Philip R DormitzerGiuseppe Del Giudice
May 29, 2012·Journal of Virological Methods·Yuzhi FuGuangqing Liu
Jul 5, 2013·Clinical Microbiology Reviews·Sook-San Wong, Richard J Webby
Jul 6, 2014·Avian Pathology : Journal of the W.V.P.A·István MészárosZoltán Zádori
Apr 1, 2017·Journal of Microscopy and Ultrastructure·Ahmed Aldarmahi
Aug 3, 2017·Animal Cells and Systems·Qian LiWeijun Guan

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Datasets Mentioned

BETA
KC996729.1

Methods Mentioned

BETA
transfection
flow cytometry
PCR
enzyme-linked immunosorbent assay

Software Mentioned

LightCycler ® 480II
ModFit LTTM

Related Concepts