Establishment and identification of the human multi-drug-resistant cholangiocarcinoma cell line QBC939/ADM.

Molecular Biology Reports
Zhi-Hua LiuHuanlong Qin

Abstract

In this study, we aim to establish the human multi-drug-resistant cholangiocarcinoma cell line QBC939/ADM which can be grow and passaged steadily in 1 μg/ml concentration of adriamycin in appropriate medium. The human multi-drug-resistant cholangiocarcinoma cell line QBC939/ADM was established using the method of exposure to medium with adriamycin alternated between high and low concentration with gradually increasing concentration. Furthermore, QBC939 and QBC939/ADM were both treated with adriamycin, mitomycin and vindesine, and then detected by MTT assay, respectively. Growth cycle and intra-cellular concentrations of ADM within cells of each group were determined by flow cytometry. Expression levels of P-glycoprotein were detected by Western bolt and real-time PCR. Results showed that, compared with QBC939, the inhibitive rates of adriamycin, mitomycin and vindesine to QBC939/ADM were lower. Content of ADM in the QBC939/ADM was lower. Western bolt and real time PCR showed that P-glycoprotein in the QBC939/ADM group was over expressed. Therefore, QBC939/ADM was establish and identified as the multi-drug-resistant cell line, which can grow and be passaged steadily in 1 μg/ml concentration adriamycin in appropriate medium. And ...Continue Reading

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Citations

Mar 10, 2019·Liver International : Official Journal of the International Association for the Study of the Liver·Silvestre VicentVincenzo Cardinale
Jan 30, 2015·World Journal of Gastroenterology : WJG·Gui-Li HuangQing-Xi Chen
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May 12, 2021·Chemico-biological Interactions·Tatyana GrigorevaViacheslav Tribulovich

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