Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2.

Emerging Microbes & Infections
Jianhui NieYouchun Wang


Pseudoviruses are useful virological tools because of their safety and versatility, especially for emerging and re-emerging viruses. Due to its high pathogenicity and infectivity and the lack of effective vaccines and therapeutics, live SARS-CoV-2 has to be handled under biosafety level 3 conditions, which has hindered the development of vaccines and therapeutics. Based on a VSV pseudovirus production system, a pseudovirus-based neutralization assay has been developed for evaluating neutralizing antibodies against SARS-CoV-2 in biosafety level 2 facilities. The key parameters for this assay were optimized, including cell types, cell numbers, virus inoculum. When tested against the SARS-CoV-2 pseudovirus, SARS-CoV-2 convalescent patient sera showed high neutralizing potency, which underscore its potential as therapeutics. The limit of detection for this assay was determined as 22.1 and 43.2 for human and mouse serum samples respectively using a panel of 120 negative samples. The cutoff values were set as 30 and 50 for human and mouse serum samples, respectively. This assay showed relatively low coefficient of variations with 15.9% and 16.2% for the intra- and inter-assay analyses respectively. Taken together, we established a ro...Continue Reading


Jul 19, 2005·Emerging Infectious Diseases·Shibo JiangShuwen Liu
Feb 10, 2009·Nature Reviews. Microbiology·Lanying DuShibo Jiang
Jun 23, 2009·Expert Review of Vaccines·Rachel L Roper, Kristina E Rehm
Oct 8, 2016·Advances in Virus Research·L EnjuanesI Sola
May 10, 2017·Human Vaccines & Immunotherapeutics·Li ZhangYouchun Wang
Dec 9, 2017·Reviews in Medical Virology·Qianqian LiYouchun Wang
Dec 10, 2017·Current Opinion in Biotechnology·Chih-Chung KuoNathan E Lewis
Mar 15, 2019·Emerging Microbes & Infections·Jianhui NieYouchun Wang
Jun 7, 2019·Human Vaccines & Immunotherapeutics·Jian MaXiaoming Yang
Dec 21, 2019·Emerging Microbes & Infections·Jianhui NieYouchun Wang
Jan 19, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·David S HuiEskild Petersen
Jan 23, 2020·Journal of Medical Virology·Yu ChenDeyin Guo
Jan 25, 2020·The New England Journal of Medicine·Na ZhuUNKNOWN China Novel Coronavirus Investigating and Research Team
Jan 30, 2020·The New England Journal of Medicine·Qun LiZijian Feng
Jan 31, 2020·JAMA : the Journal of the American Medical Association·Alexandra L PhelanLawrence O Gostin

❮ Previous
Next ❯


May 21, 2020·Occupational Medicine·Joyce C ZhangAnil Adisesh
May 30, 2020·Viruses·Nadin YounesGheyath K Nasrallah
May 22, 2020·European Journal of Immunology·Huibin LvChris K P Mok
Jun 26, 2020·The Cochrane Database of Systematic Reviews·Jonathan J DeeksUNKNOWN Cochrane COVID-19 Diagnostic Test Accuracy Group
Jul 22, 2020·The Journal of Experimental Medicine·Fabian SchmidtPaul D Bieniasz
May 1, 2020·Journal of Clinical Microbiology·Elitza S TheelKamran Kadkhoda
Apr 30, 2020·Journal of Medical Virology·Hongzhou LuYi-Wei Tang
Apr 28, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Adarsh BhimrajYngve Falck-Ytter
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Roshan J D'CruzValerie B Sampson
Jun 5, 2020·Annals of Internal Medicine·Matthew P ChengJesse Papenburg
Aug 20, 2020·Cell Research·Shulong ZuCheng-Feng Qin
Aug 1, 2020·Journal of the American Society of Nephrology : JASN·Candice ClarkeMichelle Willicombe
Oct 6, 2020·APL Photonics·Maria SolerAndrea M Armani
Jul 4, 2020·Emerging Microbes & Infections·Li LiuZhiwei Chen
Sep 17, 2020·Signal Transduction and Targeted Therapy·Yongzhi Xi
Aug 29, 2020·Nature Biotechnology·Jeffrey D WhitmanAlexander Marson
Aug 15, 2020·Nature Communications·Antonio E MuruatoPei-Yong Shi
Sep 5, 2020·Signal Transduction and Targeted Therapy·Lin WangHui Zhuang
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Ruodan XuNing Li
May 22, 2020·Journal of Pharmacokinetics and Pharmacodynamics·Roberto GomeniFrançoise Bressolle-Gomeni
Oct 10, 2020·Cell and Tissue Banking·André Oliveira PaggiaroRolf Gemperli
Oct 8, 2020·Signal Transduction and Targeted Therapy·Hao ZengQuanming Zou
Nov 11, 2020·Biochemical Society Transactions·Jiayue-Clara Jiang, Yan Zhang
Nov 16, 2020·Scandinavian Journal of Immunology·Abubakar Umar AnkaGholamreza Azizi

❮ Previous
Next ❯

Datasets Mentioned


Software Mentioned

GraphPad Prism

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The New England Journal of Medicine
Xiaoxia LuChinese Pediatric Novel Coronavirus Study Team
The Veterinary Record
Shao-Lun ZhaiDan Wang
Journal of Korean Medical Science
Wan Beom ParkMyoung Don Oh
© 2021 Meta ULC. All rights reserved