Establishment, characterization and drug sensitivity of four new human soft tissue sarcoma cell lines

International Journal of Cancer. Journal International Du Cancer
W W LiJ R Bertino

Abstract

Four new cell lines were established from patients with soft tissue sarcomas. Drug sensitivity as well as genotypic characterization, which may be related to drug sensitivity in these cell lines, was determined. Karyotype, H-ras, c-myc and mutant p53 gene expression, Rb, G1- and S-phase cyclins, E2F and major cyclin/CDK inhibitors such as p16 and p21 and p-glycoprotein were analyzed using cytogenetic, Northern blot and immunological methods. Drug sensitivity was determined using growth inhibition tests. These cell lines differed in their morphology and growth rates, forming colonies in soft agar with a cloning efficiency of 4.3-13.4%, and 3 of the 4 cell lines grew in nude mice. Cytogenetic analysis of cell lines revealed highly aneuploid karyotypes. Deletion and/or translocation of chromosome 17 was seen in HS-16, HS-18 and HS-30 cells, and both copies of chromosome 13 were lost or re-arranged in the HS-18 cell line. Mutant p53 protein was present in all 4 cell lines. HS-18 cells showed no expression of the Rb protein and high levels of expression of E2F, cyclin A, cyclin E and CDK2. HS-16 expressed a higher level of cyclin D than the other 3 cell lines. p21WAF1 expression was seen in all cell lines, but p16ink4 was expressed ...Continue Reading

References

Jul 29, 1977·Science·A W Hamburger, S E Salmon
Sep 1, 1987·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J H GerlachR M Baker
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·D G JohnsonJ R Nevins
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J SlebosK R Cho
Jul 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G P Zambetti, A J Levine
Oct 1, 1993·Molecular and Cellular Biology·K HelinA Fattaey

❮ Previous
Next ❯

Citations

May 16, 2002·American Journal of Veterinary Research·Nilanjana BanerjiSagarika Kanjilal
Jul 1, 1997·Expert Opinion on Investigational Drugs·D M Bollag
Oct 17, 1998·Cancer Investigation·E S CasperJ R Bertino
Dec 17, 2015·Cancer Metastasis Reviews·Yunfei LiaoZhenfeng Duan
Apr 26, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Roman MuffBruno Fuchs
Jan 11, 2000·Clinical Orthopaedics and Related Research·W GuoJ H Healey
May 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Donnellan, R Chetty
Mar 5, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·William M SpeesJason A Koutcher
Nov 21, 1998·Biochimica Et Biophysica Acta·M Ruas, G Peters

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.