Establishment, characterization, virus susceptibility and transfection of cell lines from cobia, Rachycentron canadum (L.), brain and fin

Journal of Fish Diseases
T-C ChengM-S Su

Abstract

Establishment and characterization of two cobia, Rachycentron canadum, cell lines derived from cobia brain (CB) and cobia fin (CF) are described. Caudal fin and brain from juvenile cobia were dissociated for 30 and 10 min, respectively, in phosphate-buffered saline containing 0.25% trypsin at 25 degrees C. The optimal culture condition for both dissociated cells (primary cell culture) was at 28 degrees C in Leibovitz-15 medium containing 10% foetal bovine serum. The cells have been sub-cultured at a ratio of 1:2 for more than 160 passages over a period of 3 years. Origin of the cultured cells was verified by comparison of their sequences of mitochondrial cytochrome oxidase subunit I genes (cox I) with the cox 1 sequence from cobia muscle tissue. The cell lines showed polyploidy. No mycoplasma contamination was detected. Susceptibility to grouper iridovirus was observed for the CB cell line but not the CF cell line. Both cell lines expressed green fluorescent protein after being transfected with green fluorescent reporter gene driven by the cytomegalovirus promoter.

References

Jan 1, 1992·Thrombosis Research·C L ZhangA Nordøy
Jan 8, 1998·The Journal of Antimicrobial Chemotherapy·D Taylor-Robinson, C Bébéar
Apr 4, 2002·In Vitro Cellular & Developmental Biology. Animal·Cord C Uphoff, Hans G Drexler
Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Jun 25, 2005·Marine Biotechnology·Julia BéjarM Carmen Alvarez
Oct 11, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Robert D WardPaul D N Hebert
Mar 15, 2006·Diseases of Aquatic Organisms·Zhengshan Zhao, Yuanan Lu
Aug 22, 2006·Journal of Virological Methods·V ParameswaranA S Sahul Hameed

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Citations

Feb 9, 2013·Archives of Virology·V Sarath BabuA S Sahul Hameed
Jan 30, 2016·In Vitro Cellular & Developmental Biology. Animal·Pengfei LiQiwei Qin
Dec 31, 2015·In Vitro Cellular & Developmental Biology. Animal·Sophia R BlochNiels C Bols
Apr 17, 2015·In Vitro Cellular & Developmental Biology. Animal·Neeraj SoodJ K Jena
Sep 4, 2018·The Journal of Veterinary Medical Science·Wenke LiLi Han

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