Establishment of 2.5D organoid culture model using 3D bladder cancer organoid culture.

Scientific Reports
Amira AbugomaaKazuaki Sasaki

Abstract

Three-dimensional (3D) organoid culture holds great promises in cancer precision medicine. However, Matrigel and stem cell-stimulating supplements are necessary for culturing 3D organoid cells. It costs a lot of money and consumes more time and effort compared with 2D cultured cells. Therefore, the establishment of cheaper and Matrigel-free organoid culture that can maintain the characteristics of a part of 3D organoids is demanded. In the previous study, we established a dog bladder cancer (BC) 3D organoid culture system by using their urine samples. Here, we successfully isolated cells named "2.5D organoid" from multiple strains of dog BC 3D organoids using 2.5 organoid media. The cell proliferation speed of 2.5D organoids was faster than parental 3D organoid cells. The expression pattern of stem cell markers was close to 3D organoids. Injection of 2.5D organoid cells into immunodeficient mice formed tumors and showed the histopathological characteristics of urothelial carcinoma similar to the injection of dog BC 3D organoids. The 2.5D organoids had a similar sensitivity profile for anti-cancer drug treatment to their parental 3D organoids. These data suggest that our established 2.5D organoid culture method might become a re...Continue Reading

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Citations

Dec 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuli ZhangSimon D Tran
Dec 29, 2020·Frontiers in Immunology·Nicolas BoucheritDaniel Olive
Feb 1, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mohammad AbbasJeffrey A Stuart
Mar 9, 2021·Developmental Biology·Ensieh ZahmatkeshMassoud Vosough
May 27, 2021·Cancer Biology & Therapy·Mohamed ElbadawyKazuaki Sasaki
Jun 22, 2021·Advanced Drug Delivery Reviews·Zhimin LuoAli Khademhosseini
Aug 20, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohamed ElbadawyKazuaki Sasaki

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Methods Mentioned

BETA
PCR
xenograft

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