Abstract
Canine mammary tumors are the most common neoplasms in intact female dogs. The surgery cannot always solve the problem, chemotherapy are recommend to these patients. However, chemotherapy could always fail because of multidrug resistance (MDR). Through stepwise increasing 5-Fluorouracil (5-FU) concentration in the culture medium, a 5-FU-resistant canine mammary tumor cell line CMT7364/5-FU was established to disclose the molecular mechanism of the drug resistance. Cell morphology, cell sensitivity to drugs, growth curves, expression of proteins, and chemo-sensitivity in vivo were compared between the parental cell line and resistant cell line. As compared it to its parental cell line (CMT7364), CMT7364/5-FU showed different morphology, cross-resistant to other chemo-drugs and a prolonged population doubling time (PDT). The drug efflux pump proteins (ABCB1 and ABCG2) in CMT7364/5-FU were up-regulated. In vivo, the similar result revealed that CMT7364/5-FU cell line was more resistant to 5-FU. In conclusion, a 5-FU-resistant canine mammary tumor cell line (CMT7364/5-FU) was successfully established, it can serve as a good model for researching the mechanism of MDR and screening effective agents to reverse drug resistance.
References
Nov 11, 1976·Biochimica Et Biophysica Acta·R L Juliano, V Ling
Jan 1, 1991·British Journal of Cancer·K Snow, W Judd
May 1, 1995·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·R LaskawiA Schott
Feb 14, 1998·Journal of Natural Products·S E KimY C Kim
Apr 13, 1999·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Y Ikezawa, R B Gustilo
Apr 24, 2001·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·M KarayannopoulouA Dessiris
Jul 11, 2002·Cancer Chemotherapy and Pharmacology·Iwao IwasakiHiromichi Hemmi
May 2, 2003·Nature Reviews. Cancer·Daniel B LongleyPatrick G Johnston
Oct 25, 2003·Oncogene·L Austin Doyle, Douglas D Ross
Dec 5, 2003·Journal of Cancer Research and Clinical Oncology·Kaoru OkugawaHitoo Nakano
May 18, 2005·Molecular Cancer Therapeutics·Susan PrattAnne H Dantzig
Jun 15, 2005·Journal of Veterinary Pharmacology and Therapeutics·J GriffinD J Brayden
Jun 27, 2008·Cellular and Molecular Life Sciences : CMLS·H Lage
Sep 17, 2009·Reproduction in Domestic Animals = Zuchthygiene·K U HonschaG Gäbel
Dec 24, 2009·Cancer Science·Takatsugu IshimotoOsamu Nagano
Mar 13, 2010·Molecular and Cellular Biochemistry·Te LiuZhixue Liu
Jun 8, 2011·Journal of Veterinary Pharmacology and Therapeutics·K L Mealey
Oct 7, 2011·Stem Cells and Development·Nathan MooreStephen Lyle
Aug 3, 2012·Nature·Jian ChenLuis F Parada
Nov 28, 2012·Cancer Chemotherapy and Pharmacology·Consolación MelguizoAntonia Aránega
Feb 1, 2013·Journal of Veterinary Pharmacology and Therapeutics·M ZandvlietJ A Schrickx
Mar 8, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Thu Phuong TranHye Gwang Jeong
Jun 15, 2013·Cancer Cell·Alexander RoeschMeenhard Herlyn
Sep 24, 2013·Advanced Drug Delivery Reviews·Alejandro Sosnik
Apr 15, 2014·Polish Journal of Veterinary Sciences·M KrólT Motyl