Establishment of a canine model of human type 2 diabetes mellitus by overexpressing phosphoenolypyruvate carboxykinase

International Journal of Molecular Medicine
Yeon Woo JeongWoosuk Hwang

Abstract

Dogs are useful models for studying human metabolic diseases such as type 2 diabetes mellitus due to similarities in physiology, anatomy and life styles with humans. Somatic cell nuclear transfer (SCNT) facilitates the production of transgenic dogs. In this study, we generated transgenic dogs expressing the phosphoenolpyruvate carboxykinase (PEPCK) gene, which is closely involved in the pathogenesis of type 2 diabetes mellitus. In addition, we assessed the cloning efficiency associated with adult or fetal (cloned or natural mating) fibroblasts as a nuclear source. Cloning efficiency was determined by the fusion, pregnancy and cloning rates. The fusion rates were significantly high for fibroblasts from cloned fetuses, but the pregnancy and cloning rates were relatively high for cells from normal fetuses. Based on these data, fetal fibroblasts were selected as the nuclear donor for SCNT and genetically engineered to overexpress the PEPCK gene and dual selection marker genes controlled by the PEPCK promoter. The transgenic cells were introduced into oocytes and transferred into five recipient dogs, resulting in two pregnancies. Finally, three puppies were born and confirmed by microsatellite analysis to be genetically identical to...Continue Reading

References

Jan 1, 1989·International Review of Cytology·J W Gordon
Jan 1, 1986·Annual Review of Genetics·R D Palmiter, R L Brinster
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·A ValeraF Bosch
Jan 1, 1996·Critical Reviews in Eukaryotic Gene Expression·D L Sokol, A M Gewirtz
Feb 27, 1997·Nature·I WilmutK H Campbell
Jan 1, 1997·Annual Review of Biochemistry·R W Hanson, L Reshef
May 20, 1999·Nature Biotechnology·A BaguisiY Echelard
Feb 26, 2000·Trends in Genetics : TIG·E A OstranderD F Patterson
Sep 19, 2000·Nature·I A PolejaevaK H Campbell
Mar 30, 2002·Nature Biotechnology·Patrick ChesnéJean-Paul Renard
Sep 16, 2003·Cell Biology International·Gordana NikcevicMilena Stevanovic
Mar 5, 2004·Biology of Reproduction·A M PowellR J Wall
Dec 14, 2004·Theriogenology·Gaia C LuvoniDebora Macis
Aug 5, 2005·Nature·Byeong Chun LeeWoo Suk Hwang
Apr 6, 2006·Developmental Biology·Ziyi LiJohn F Engelhardt
Jul 27, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Kate L TsaiKeith E Murphy
Apr 9, 2008·Biochimie·Richard W Hanson, Parvin Hakimi
Aug 21, 2008·Nature Reviews. Genetics·Elinor K Karlsson, Kerstin Lindblad-Toh
Dec 9, 2008·Animal Reproduction Science·Mohammad Shamim HosseinWoo Suk Hwang
Jan 13, 2009·Animal Reproduction Science·So Gun HongByeong Chun Lee
Feb 11, 2009·Cloning and Stem Cells·Martha C GómezJakob Reiser
Feb 20, 2009·Cloning and Stem Cells·Mohammad Shamim HosseinWoo Suk Hwang
Jan 12, 2011·Theriogenology·Hyun Ju OhByeong Chun Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.