Establishment of a chick embryo shell-less culture system and its use to observe change in behavior caused by nicotine and substances from cigarette smoke

Toxicology Letters
S Hamamichi, Hideo Nishigori

Abstract

Early detection and removal of harmful factors are essential to the proper physical and psychological development of the fetus, presumably showing the effects during the prenatal period and after birth. As one procedure to aid in understanding such factors, we have established a shell-less culture system for video monitoring to observe change in behavior of 7-day-old chick embryos. Nicotine and aqueous cigarette smoke extract (ACSE) were selected for the present experiments, and the results showed a complete stoppage of swing-like movements by administrations of 10 microg nicotine and 1xACSE, possibly displaying paralytic symptoms. Quantitative analysis of nicotine in 1xACSE indicated that more than 10 microg of nicotine were contained in 100 microl of the extract. The present system, although in initial stage of development, may be a useful preliminary screening procedure for perhaps supervision and warning about the environment surrounding pregnant women.

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Citations

Oct 4, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hideo Nishigori
Dec 23, 2011·The Review of Diabetic Studies : RDS·Savita P Datar, Ramesh R Bhonde
Apr 5, 2013·Stem Cells International·Timo SchomannBarbara Kaltschmidt
May 15, 2016·Mechanisms of Development·Domenico Ribatti
Nov 12, 2016·Reproductive Toxicology·Domenico Ribatti
Nov 13, 2003·Journal of Biomedical Materials Research. Part a·Ulrike KluehDon L Kreutzer
Dec 12, 2003·Journal of Biomedical Materials Research. Part a·U KluehD L Kreutzer
Jun 30, 2011·Annals of Plastic Surgery·Antoine L CarreH Peter Lorenz
Jan 5, 2008·American Journal of Respiratory and Critical Care Medicine·Saravanan RajendrasozhanIrfan Rahman
Feb 2, 2008·American Journal of Respiratory Cell and Molecular Biology·Se-Ran YangIrfan Rahman
Mar 30, 2020·Tissue Engineering. Part B, Reviews·Greet MerckxAnnelies Bronckaers

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