Establishment of a Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics Method and Its Application to Lichen Profiling

Analytical Chemistry
Hisahiro KaiKoji Matsuno

Abstract

Direct-injection electron ionization-mass spectrometry (DI-EI-MS) is a multivariate analysis method useful for characterizing biological materials. We demonstrated the use of DI-EI-MS for metabolic profiling using several closely related lichen species: Cladonia krempelhuberi, C. gracilis, C. pseudogymnopoda, and C. ramulosa. The methodology involves conversion of total ion chromatograms to integrated chromatograms and assessment of reproducibility. The qualitative DI-EI-MS method was used to profile the major and/or minor constituents in extracts of lichen samples. It was possible to distinguish each lichen sample by altering the electron energy in DI-EI-MS and examining the resulting data using one-way analysis of variance. Previously undetectable peaks, which are easy to fragment could be revealed by varying the electron energy. Our results suggest that metabolic profiling using DI-EI-MS would be useful for discriminating between subgroups within the same species. This is the first study to report the use of DI-EI-MS in a metabolomics application.

References

Dec 28, 2005·Journal of Natural Products·Kaoru KinoshitaIsao Yoshimura
Jan 21, 2009·Annual Review of Plant Biology·Kazuki Saito, Fumio Matsuda
Jun 9, 2012·Phytochemical Analysis : PCA·Haidy A GadMohamed M Al-Azizi
Mar 29, 2014·Natural Product Reports·Madeleine ErnstNorberto Peporine Lopes
Jul 3, 2015·Journal of Natural Products·Kaoru KinoshitaKunio Takahashi
Jan 17, 2016·Annual Review of Food Science and Technology·Suzanne D JohanningsmeierClaire M Klevorn
Apr 30, 2016·International Journal of Molecular Sciences·Ina Aretz, David Meierhofer

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