Establishment of a genetic transformation system for the marine pennate diatom Fistulifera sp. strain JPCC DA0580--a high triglyceride producer.

Marine Biotechnology
Masaki MutoTsuyoshi Tanaka

Abstract

A genetic transformation system for the marine pennate diatom, Fistulifera sp. JPCC DA0580, was established using microparticle bombardment methods. Strain JPCC DA0580 has been recently identified as the highest triglyceride (60 % w/w) producer from a culture collection of 1,393 strains of marine microalgae, and it is expected to be a feasible source of biodiesel fuel. The transformation conditions for strain JPCC DA0580 were optimised using the green fluorescent protein gene (gfp) and the gene encoding neomycin phosphotransferase II (nptII). The most efficient rate of transformation was attained when tungsten particles (0.6 μm in diameter) were used for microparticle bombardment. The effect of endogenous and exogenous promoters on the expression of nptII was examined. Endogenous promoters were more efficient for obtaining transformants compared with exogenous promoters. Southern hybridisation analysis suggested that nptII integrated into the nuclear genome. This genetic manipulation technique should allow us to understand the mechanisms of high triglyceride accumulation in this strain, thereby contributing to improving BDF production.

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Citations

Nov 19, 2013·Marine Drugs·Heni AbidaChris Bowler
May 6, 2015·Marine Drugs·Vandana VinayakBenoît Schoefs
Jan 16, 2016·Biotechnology for Biofuels·Yoshiaki MaedaTsuyoshi Tanaka
Jul 22, 2014·Journal of Bioscience and Bioengineering·Yoshihiko SunagaTsuyoshi Tanaka
Jan 1, 2013·Metabolites·Toshihiro ObataAdriano Nunes-Nesi
Mar 11, 2018·Scientific Reports·Willem StockWim Vyverman
Jul 19, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Weichao Huang, Fayza Daboussi
Jul 19, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Olga SayanovaJohnathan A Napier
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Gulshan KumarTilak Raj Sharma
Oct 30, 2019·Critical Reviews in Food Science and Nutrition·Zhi-Cong LiangJian-Guo Jiang
Apr 17, 2021·Metabolic Engineering·Yuichiro KashiyamaTsuyoshi Tanaka
Apr 15, 2021·Biotechnology and Bioengineering·Yoshiaki MaedaTsuyoshi Tanaka
Aug 6, 2017·Progress in Lipid Research·Lu-Lu XueJian-Guo Jiang
Jul 25, 2013·Journal of Proteome Research·Daisuke NojimaTsuyoshi Tanaka
Jul 30, 2014·Analytical Chemistry·Masahito HosokawaTsuyoshi Tanaka

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