Establishment of a hepatocyte cell line producing growth-promoting factors for liver-colonizing tumor cells

Japanese Journal of Cancer Research : Gann
T YamoriT Tsuruo

Abstract

A hepatocyte-derived cell line designated MLE-15A2 was established from a primary culture of mouse hepatocytes. The MLE-15A2 cells appeared to retain the basic nature of hepatocytes in that they showed morphology of an epithelial cell type and secreted albumin into the culture medium. These cells were grown on collagen-coated plates and could be easily expanded to a large-scale culture. Therefore, MLE-15A2 cells may provide a more useful model for studying liver microenvironments than primary cultures of hepatocytes. We found that conditioned media from MLE-15A2 cells, as well as from primary cultures of hepatocytes, promoted the proliferation of highly liver-colonizing colon 26 NL-17 cells better than the poorly liver-colonizing colon 26 NL-4 cells. Moreover, the conditioned media stimulated the growth of some human colon cancer cell lines. These results indicate that MLE-15A2 cells secrete growth factors that selectively stimulate certain tumor cell types. Hepatocyte-derived growth factors may regulate selective survival and colonization of tumor cells in the process of liver metastasis. The growth-promoting activity was unaffected by dialysis, was stable at 80 degrees C for 30 minutes and was bound to a heparin-Sepharose col...Continue Reading

References

Feb 1, 1990·Cancer Metastasis Reviews·P N Belloni, R J Tressler
Mar 1, 1990·The New England Journal of Medicine·B R Zetter
Jan 1, 1989·Japanese Journal of Cancer Research : Gann·M KoenumaT Tsuruo
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Jun 8, 1984·Science·P A Netland, B R Zetter
Jan 1, 1982·Cancer Metastasis Reviews·I R Hart

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Citations

Dec 21, 2000·Japanese Journal of Cancer Research : Gann·S ShimizuK Hirakawa
Jan 23, 1999·Japanese Journal of Cancer Research : Gann·T OgoshiS Matsuno
Nov 6, 1998·Nature·A R Means

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