PMID: 9436858Jan 22, 1998Paper

Establishment of a human pancreatic tumor xenograft model: potential application for preclinical evaluation of novel therapeutic agents

Pancreas
R M MohammadF H Sarkar

Abstract

Adenocarcinoma of the pancreas is currently the fifth leading cause of death in the United States. It remains generally incurable by available treatment modalities. We report here on the characterization of a permanent pancreatic cell line (KCI-MOH1), established as a xenograft in severe combined immune deficient (SCID) mice, from a 74 year-old African American male patient diagnosed with pancreatic cancer. Sections from paraffin-embedded tumors excised from SCID mice revealed typical adenocarcinoma of the pancreas. Karyotypic analysis of cultured cells derived from tumors grown in SCID mice revealed a male karyotype with multiple clonal aberrations: 42, XY, add (3)(p11.2), der(7) t(7;12) (p22;q12), -10, -12, add (14)(p11), -18, add (20)(q13)-22/84, idemx2. Immunostaining of KCI-MOH1 tissues shows strong expression of p53 and p21 proteins. The xenograft model was established by transplanting the KCI-MOH1 cells subcutaneously (s.c.) in SCID mice. When the s.c. tumor was transplanted in vivo to other SCID mice, the success rate was 100%, with a doubling time of 8.5 days. The SCID mouse xenograft model was used to test the efficacy of selected standard chemotherapeutic drugs (taxol, gemcitabine, 5-fluorouracil, and Ara-C) and nove...Continue Reading

Citations

Jun 10, 2005·Journal of Translational Medicine·Bonnie L HylanderElizabeth A Repasky
Mar 29, 2003·Expert Opinion on Biological Therapy·Lawrence Rosenberg, Mark Lipsett
Jun 6, 2008·International Journal of Cancer. Journal International Du Cancer·Zhiwei WangRamzi M Mohammad
Mar 18, 2006·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Juergen TepelHolger Kalthoff
Jan 24, 2012·Cell·Andrew D RhimBen Z Stanger
Apr 21, 2016·Cancers·Daniel ZeitouniKirsten L Bryant
Jun 19, 2004·Leukemia Research·Claire QuineyChristian Billard
May 8, 2015·Cell Death & Disease·S MahaleB Chaudhuri
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·P MukherjeeS J Gendler
Jan 23, 2004·Cancer Investigation·Jeremy Kortmansky, Gary K Schwartz
Jan 10, 2018·Cold Spring Harbor Perspectives in Medicine·Ian AhearnMark R Philips
Feb 28, 2008·Journal of Natural Products·Sanjeev BanerjeeRamzi M Mohammad
Jan 17, 2016·Genes & Development·Shigetsugu TakanoAnil K Rustgi

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