Establishment of a method for in-vivo SPECT/CT imaging analysis of 111 In-labeled exendin-4 pancreatic uptake in mice without the need for nephrectomy or a secondary probe

Nuclear Medicine and Biology
Keita HamamatsuNobuya Inagaki

Abstract

Radiolabeled exendin derivatives have been developed to visualize and quantify pancreatic beta cells. However, there are currently no established methods for analyzing in-vivo SPECT/CT images to quantify probe accumulation in the pancreas in rodent models. In this study, we aimed to establish an analytical method for murine in-vivo SPECT/CT imaging. First, we investigated the correlation between radioactivity measured by curiemeter and uptake calculated from SPECT/CT images of pancreata harvested after probe injection. Second, ROI volume necessary for reliable estimation of pancreatic uptake value was also examined. Third, the influence of high renal uptake on analysis was investigated with SPECT/CT imaging of harvested kidneys. Fourth, we compared pancreatic uptake values and ROI volumes estimated from in-vivo SPECT/CT images of pre- and post-nephrectomy mice. Finally, we assessed the correlation between the pancreatic uptake values from in-vivo SPECT/CT image analysis and radioactivity of harvested pancreata determined with a curiemeter. Radioactivity of harvested pancreata measured by curiemeter and uptake values derived from SPECT/CT imaging of harvested pancreas showed an almost perfect correlation (r = 0.99, p < 0.001). A...Continue Reading

References

Dec 28, 2002·Diabetes·Alexandra E ButlerPeter C Butler
Jun 26, 2007·Biophysical Journal·Junghyo JoDuk-Su Koh
Sep 10, 2009·Biochemical and Biophysical Research Communications·Eri MukaiNobuya Inagaki
Jul 30, 2014·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Iris MathijsLuc Bouwens
Feb 25, 2017·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Martin A Lodge

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Citations

Feb 14, 2020·Endocrinology·Daniela Nasteska, David J Hodson
Aug 3, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Naotaka FujitaNobuya Inagaki
Apr 12, 2021·Drug Discovery Today·Seyed Ebrahim Alavi, Hasan Ebrahimi Shahmabadi
Jul 13, 2021·Frontiers in Endocrinology·Takaaki MurakamiNobuya Inagaki
Mar 24, 2020·Molecular Pharmaceutics·Hiroyuki WatanabeMasahiro Ono

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