Establishment of a method for measuring total complement activity based on a hemolysis system using own red blood cells

Journal of Immunological Methods
Ruirui Dong, Hui Liu

Abstract

To establish a simple, stable method for measuring total complement activity in plasma. Total complement activity (TCA) of plasma was measured using a classical method (CH50 method) and a self-hemolysis colorimetric method (new method). Human red blood cells (RBCs) were used as a hemolysis indicator system and rabbit-anti-human RBC antibody instead of the traditional hemolysin (rabbit-anti-sheep RBC antibody) in the new method. TCA in intensive care unit (ICU) patients and healthy individuals was measured using the new method. TCA using the new method and the CH50 method were significantly correlated. TCA in ICU patients was significantly lower than that in healthy individuals. The self-hemolysis colorimetric method is a simple and stable method, and has potential value in clinical applications.

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Citations

Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Chen YuLian Li

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