Establishment of a nasopharyngeal carcinoma cell line capable of undergoing lytic Epstein-Barr virus reactivation

Laboratory Investigation; a Journal of Technical Methods and Pathology
Yim Ling YipSai Wah Tsao

Abstract

Epstein-Barr virus (EBV) infects more than 90% of the adult human population. Undifferentiated nasopharyngeal carcinoma (NPC) is common in Southeast Asia, with a particularly high incidence among southern Chinese. The EBV genome can be detected in practically all cancer cells in undifferentiated NPC. The role of EBV in pathogenesis of undifferentiated NPC remains elusive. NPC cell lines are known to be difficult to establish in culture. The EBV+ve NPC cell lines, even if established in culture, rapidly lost their EBV episomes upon prolonged propagation. At present, the C666-1 NPC cell line, which is defective in lytic EBV reactivation, is the only EBV+ve NPC cell line available for NPC and EBV research. The need to establish new and representative NPC cell lines is eminent for NPC and EBV research. In this study, we report the use of the Rho-associated kinase inhibitor (Y-27632) has facilitated the establishment of a new EBV+ve NPC cell line from an earlier established NPC xenograft, C17. The C17 cell line was tumorigenic in immune-deficient mice (NOD/SCID). It retained the EBV episomes and could be induced to undergo productive lytic reactivation of EBV to generate infectious virus particles. The C17 cell line represents a new...Continue Reading

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Citations

Oct 5, 2019·PLoS Pathogens·Rachel Hood EdwardsNancy Raab-Traub
Apr 7, 2020·Expert Opinion on Therapeutic Targets·Valentin BalochePierre Busson
Jun 2, 2020·Journal of Medical Virology·Xuefeng Liu, Abdul M Mondal
Oct 20, 2020·Journal of Cellular and Molecular Medicine·Raymond Wai-Ming LungKa-Fai To
Oct 24, 2020·Signal Transduction and Targeted Therapy·Yuanbo KangLei Wang
Mar 7, 2021·Viruses·Yi Tian PngChwee Ming Lim
May 31, 2021·Tumour Virus Research·Lori Frappier
Dec 5, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Jingjing ShiPek-Lan Khong

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Methods Mentioned

BETA
xenograft
biopsies
PCR
xenografts
transfection

Software Mentioned

SKY View

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