Establishment of a new bovine leukosis virus producing cell line

Journal of Virological Methods
D BeierO Marquardt

Abstract

Due to the prevalence of different bovine leukosis virus (BLV) species in the cattle population in Europe, problems may arise in the serological diagnosis of BLV infections. In addition, earlier investigations demonstrated that contamination of the BLV antigen-producing cell culture systems by bovine viral diarrhea virus (BVDV) may give rise to misinterpretation of serological test results after BVDV vaccination of cattle. By co-cultivation of peripheral leukocytes of a BLV-infected cow with a permanent sheep kidney cell line, a new BLV-producing cell line named PO714 was established. This line carries a BLV provirus of the Belgian species and has been tested to be free of a variety of possibly contaminating viruses and mycoplasms. Investigations of a panel of well-characterised sera by agar gel immunodiffusion (AGID) and capture ELISA (cELISA) tests using antigen prepared from this new cell line in comparison with antigen of the well-known cell line FLK/BLV yielded comparable results. False positive results caused by BVDV cross-reactions could be eliminated when tests were carried out with antigen derived from the new cell line.

References

Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N SagataY Ikawa
Jul 1, 1994·Journal of Virological Methods·S R BolinR Roblin
Nov 1, 1995·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·H J WagnerS Risse
Jun 1, 1996·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·E MolteniE Ginelli
Dec 1, 1996·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·H FechnerD Beier

❮ Previous
Next ❯

Citations

Nov 8, 2011·Genetics and Molecular Research : GMR·M R MohammadabadiM Honarmand
Jul 31, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Stephanie GrossJörg Andrä
Jul 19, 2013·Journal of Immunoassay & Immunochemistry·Clarissa C de CastroSilvia de O Hübner

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY062265

Methods Mentioned

BETA
ELISA
PCR
FCS
electron microscopy
cELISA

Software Mentioned

DNASIS

Related Concepts