Establishment of a novel human embryonic stem cell-derived trophoblastic spheroid implantation model

Human Reproduction
Yin-Lau LeeKai-Fai Lee

Abstract

Can human embryonic stem cell-derived trophoblastic spheroids be used to study the early stages of implantation? We generated a novel human embryonic stem cell-derived trophoblastic spheroid model mimicking human blastocysts in the early stages of implantation. Both human embryos and choriocarcinoma cell line derived spheroids can attach onto endometrial cells and are used as models to study the early stages of implantation. However, human embryos are limited and the use of cancer cell lines for spheroid generation remains sub-optimal for research. Experimental induced differentiation of human embryonic stem cells into trophoblast and characterization of the trophoblast. Trophoblastic spheroids (BAP-EB) were generated by inducing differentiation of a human embryonic stem cell line, VAL3 cells with bone morphogenic factor-4, A83-01 (a TGF-β inhibitor), and PD173074 (a FGF receptor-3 inhibitor) after embryoid body formation. The expressions of trophoblastic markers and hCG levels were studied by real-time PCR and immunohistochemistry. BAP-EB attachment and invasion assays were performed on different cell lines and primary endometrial cells. After 48 h of induced differentiation, the BAP-EB resembled early implanting human embryos...Continue Reading

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Citations

Sep 4, 2016·Human Reproduction Update·Teena Kjb GamageJoanna L James
Oct 1, 2016·Politics and the Life Sciences : the Journal of the Association for Politics and the Life Sciences·Roman Anton
Jul 28, 2020·Human Reproduction Update·Ana M Pereira DaoudBjörn Heindryckx
Nov 30, 2019·Journal of Assisted Reproduction and Genetics·Jemma EvansLois A Salamonsen
Jan 4, 2017·Journal of Cell Science·John D Aplin, Peter T Ruane
Dec 13, 2019·Scientific Reports·Houzhu DingRobert C Chang
Apr 30, 2021·Acta Biomaterialia·Anna StejskalováBenjamin D Almquist
Apr 27, 2021·Biology of Reproduction·J ZhouD J Schust
Aug 6, 2021·Frontiers in Veterinary Science·Sharon ArcuriTiziana A L Brevini

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