PMID: 16514177Mar 4, 2006Paper

Establishment of a primary culture model of mouse uterine and vaginal stroma for studying in vitro estrogen effects

Experimental Biology and Medicine
Keiko InadaT Sato

Abstract

Effects of 17beta-estradiol (E2) on uterine and vaginal epithelial cell proliferation could be mediated by stromal cell-derived paracrine factors. To study the epithelial-stromal interactions in mice, an in vitro model of uterine and vaginal stromal cells of immature mice is essential. Therefore, we established a primary culture model of stromal cells both from uterus and vagina and examined the effect of E2 on proliferation of cultured stromal cells. We found that E2 stimulated proliferation of stromal cells from both organs in vitro, showing an increase in the number of cells and the percentage of 5-bromo-2'-deoxyuridine (BrdU)-labeled cells. Interestingly, vaginal stromal cells responded to lower E2 than uterine stromal cells in proliferation (10(-12) M vs. 10(-8) M) and BrdU labeling (10(-14) - 10(-10) M vs. 10(-10) - 10(-6) M). To examine the effect of E2 in vivo, cells were grafted into the subrenal capsule of the host mice and grown for 2 weeks. The BrdU labeling in cultured stromal cells was increased by E2 in vivo. To examine the effect of cultured stromal cells on epithelial cell proliferation, uterine and vaginal epithelium of adult mice were separated, recombined with the cultured stromal cells, and grafted under th...Continue Reading

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Citations

Jan 24, 2008·Immunologic Research·Matthew M Seavey, Tim R Mosmann
Dec 4, 2009·Environmental Health and Preventive Medicine·Hong ZhouPei-Yu Wang
Apr 18, 2013·Molecular Endocrinology·Yanping ZhangL Bruno Ruest
Aug 5, 2015·The Journal of Infectious Diseases·Sandra LasarteMiguel Relloso
Mar 7, 2015·Nature Reviews. Immunology·Charles R WiraMickey V Patel
Sep 16, 2020·Cancer Treatment Reviews·Anita SemertzidouMaria Kyrgiou
Nov 16, 2020·Differentiation; Research in Biological Diversity·Taisen IguchiNoboru Takasugi
Apr 8, 2021·Reproductive Sciences·Jennifer M McCrackenJulie C E Hakim

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