Establishment of a prostatic small-cell carcinoma cell line (SO-MI)

The Prostate
Hiroshi OkadaS Kamidono

Abstract

Prostatic small-cell carcinoma is an extremely rare, highly aggressive disease. We established a cell line from this tumor. Tumor tissue obtained from a 24-year-old Japanese man was used to establish the cell line. Cultured cells and tumors transplanted into nude mice were characterized by histologic, immunohistologic, immunocytologic, and molecular biologic methods. An immortal culture cell line (SO-MI) was successfully established. SO-MI cells adhered weakly to plastic surfaces in vitro, showing a 52- to 72-hr doubling time. SO-MI cells were heterotopically and orthotopically transplantable in nude mice. The cells were immunoreactive for NSE, chromogranin A, and NCAM, but not for ACTH, calcitonin, serotonin, gastrin, insulin, glucagons, LCA, EMA, PAP, PSA, androgen receptor, and p53. SO-MI cells secreted NSE in vitro and in vivo. SO-MI cells at passage 30 contained 50-59 chromosomes with a modal number of 55. PCR suggested that the p53 gene was deleted in SO-MI cells. RT-PCR detected no mRNA encoding androgen receptor in these cells. SO-MI cells retain the neuroendocrine nature of the original tumor, and should be useful in studying possible etiologies and new treatments.

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Citations

May 26, 2006·The American Journal of Surgical Pathology·Jorge L YaoP Anthony di Sant'Agnese
Oct 1, 2003·The Prostate·Adrie van BokhovenM Scott Lucia
Oct 1, 2003·The Prostate·Adrie van BokhovenMarileila Varella-Garcia

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