Establishment of a proteomic profile associated with gonocyte and spermatogonial stem cell maturation and differentiation in neonatal mice

Proteomics
Bo ZhengXiaoyan Huang

Abstract

Initiation of the first wave of spermatogenesis in the neonatal mouse testis is characterized by differentiation of a transient population of germ cells called gonocytes in the center of the seminiferous tubules. After resuming mitotic activity, gonocytes relocate on the basement membrane, giving rise to spermatogonial stem cells (SSCs). These processes begin from birth in mice, and differentiated type A spermatogonia first appear by day 6 postpartum. During these processes, Sertoli cells within the seminiferous tubules and Leydig cells in the interstitial tissue form the stem cell "niche," and influence SSC fate decisions. Thus, we collected whole mouse testis tissues during the first wave of spermatogenesis at specific time points (days 0.5, 1.5, 2.5, 3.5, 4.5, and 5.5 postpartum) and constructed a comparative proteomic profile. We identified 252 differentially expressed proteins classified into three clusters based on expression, and bioinformatics analysis correlated each protein pattern to specific cell processes. Expression patterns of nine selected proteins were verified via Western blot, and cellular localizations of three proteins with little known information in testes were further investigated during spermatogenesis....Continue Reading

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Citations

Oct 16, 2015·Human Molecular Genetics·Yu KangXingxu Huang
Mar 11, 2015·Journal of Proteomics·Binbin ShaoJiahao Sha
Aug 15, 2017·Reproduction in Domestic Animals = Zuchthygiene·P-F ZhangM Zhang
Feb 12, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Gurpreet Manku, Martine Culty
Aug 20, 2019·Proteomics. Clinical Applications·Yueshuai GuoXuejiang Guo
May 11, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Bo ZhengXiaoyan Huang
May 1, 2021·Frontiers in Cell and Developmental Biology·Jun YuFei Sun

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