Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone

Osteoarthritis and Cartilage
Y LiY Xia

Abstract

To investigate the role of advanced glycation end products (AGEs) in cartilage degeneration in vivo and determine the influence of the peroxisome proliferator-activated receptor-γ (PPARγ) agonist pioglitazone on AGEs-induced osteoarthritis (OA) in a rabbit model. Thirty-two rabbits were separated into four groups (n = 8 each) and received 500 μL of 123, 350, or 1000 mmol/L D-ribose or Phosphate buffered saline (PBS) solution administered to the right stifle joint via intra-articular injection twice a week. All the rabbits ran 500 m on treadmills every day. Another 16 rabbits were administered 1000 mmol/L D-ribose and divided into 2 groups (n = 8) that received either placebo or pioglitazone administered orally at 20 mg/kg/day. Eight weeks later, cartilage damage was evaluated macroscopically, histologically, and biochemically. Artificially increasing the AGEs level and exercise load resulted in cartilage damage and dose-dependent downregulation of PPARγ expression. The efficacy of pioglitazone treatment was tested in a rabbit OA model, and a clear chondroprotective effect was revealed by macro- and microscopic assessments. Elevating AGEs in rabbits can accelerate the articular cartilage degradation that occurs with physical exe...Continue Reading

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Citations

May 20, 2016·Nature Reviews. Rheumatology·Richard F LoeserBrian O Diekman
Feb 18, 2018·Cell Biology International·Zhao-Jun WangJian-Xia Liang
Jul 19, 2019·Cell Biochemistry and Function·Asma Shaikh-KaderHeidi Abrahamse
Aug 16, 2016·Apoptosis : an International Journal on Programmed Cell Death·Hai-Bin ZhangZhao-Jun Wang
Jul 31, 2019·Chemical Reviews·Pei-Long WangHong-Cai Zhou

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