Establishment of a root proteome reference map for the model legume Medicago truncatula using the expressed sequence tag database for peptide mass fingerprinting

Proteomics
Ulrike MathesiusBarry G Rolfe

Abstract

We have established a proteome reference map for Medicago truncatula root proteins using two-dimensional gel electrophoresis combined with peptide mass fingerprinting to aid the dissection of nodulation and root developmental pathways by proteome analysis. M. truncatula has been chosen as a model legume for the study of nodulation-related genes and proteins. Over 2,500 root proteins could be displayed reproducibly across an isoelectric focussing range of 4-7. We analysed 485 proteins by peptide mass fingerprinting, and 179 of those were identified by matching against the current M. truncatula expressed sequence tag (EST) database containing DNA sequences of approximately 105,000 ESTs. Matching the EST sequences to available plant DNA sequences by BLAST searches enabled us to predict protein function. The use of the EST database for peptide identification is discussed. The majority of identified proteins were metabolic enzymes and stress response proteins, and 44% of proteins occurred as isoforms, a result that could not have been predicted from sequencing data alone. We identified two nodulins in uninoculated root tissue, supporting evidence for a role of nodulins in normal plant development. This proteome map will be updated c...Continue Reading

Citations

Jul 17, 2008·Comparative and Functional Genomics·Barry G RolfeW Dietz Bauer
Nov 30, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Thomas C HunterPaul A Haynes
Mar 9, 2007·Bioinformatics·Ewa Urbanczyk-Wochniak, Lloyd W Sumner
Jun 11, 2003·Molecular Plant-microbe Interactions : MPMI·Michael A DjordjevicUNKNOWN Sinorhizobium DNA Sequencing Consortium
Mar 20, 2003·Plant Physiology·Bonnie S WatsonLloyd W Sumner
Nov 28, 2008·Plant Physiology·Laurent BrechenmacherGary Stacey
Dec 11, 2007·Applied and Environmental Microbiology·Oscar MaestreJuan C Mauricio
Nov 26, 2009·Canadian Journal of Microbiology·Francine M Perrine-WalkerCharles H Hocart
Jan 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ulrike MathesiusWolfgang D Bauer
Feb 22, 2008·International Journal of Plant Genomics·Jean-Michel AnéJulia Frugoli
May 27, 2005·Molecular Plant-microbe Interactions : MPMI·Jinrong WanGary Stacey
Sep 1, 2006·Molecular Plant-microbe Interactions : MPMI·Nardjis AmiourEliane Dumas-Gaudot
Jul 31, 2007·Journal of Plant Physiology·M Irfan QureshiLello Zolla
Aug 30, 2005·Proteomics·Jean-Marc GionChristophe Plomion
Jun 11, 2005·Biotechnology and Bioengineering·Anne IncampsEric Quéméneur
Jun 6, 2009·The New Phytologist·Michael W ShaneHans Lambers
Jul 11, 2008·Journal of Separation Science·Wei WangShaoning Chen
Dec 6, 2006·Proteomics·Haibin XuZhengqiang Ma
Mar 18, 2006·Proteomics·Francisco Javier Fernández-AceroJesús Jorrín
Jan 22, 2011·Electrophoresis·Junbo XiongWang Ding
May 5, 2016·Biochemistry Research International·Pramod Kumar SinghSameer S Bhagyawant
Jul 8, 2016·International Journal of Molecular Sciences·Giel E van NoordenUlrike Mathesius
Aug 25, 2016·Frontiers in Plant Science·Yun KangJerome Verdier
Jul 9, 2002·Phytochemical Analysis : PCA

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.