Establishment of a Somatic Cell Bank for Indian Buffalo Breeds and Assessing the Suitability of the Cryopreserved Cells for Somatic Cell Nuclear Transfer

Cellular Reprogramming
Naresh L SelokarPrem Singh Yadav

Abstract

Biobanks of cryopreserved gametes and embryos of domestic animals have been utilized to spread desired genotypes and to conserve the animal germplasm of endangered breeds. In principle, somatic cells can be used for the same purposes, and for reviving of animals, the somatic cells must be suitable for animal cloning techniques, such as somatic cell nuclear transfer. In the present study, we derived and cryopreserved somatic cells from three breeds of riverine and swamp-like type buffaloes and established a somatic cell bank. In total, 350 cryovials of 14 different individual animals (25 cryovials per animal) were cryopreserved and informative data such as breed value, origin, and others were documented. Immunostaining of the established cells against vimentin and cytokeratin suggested a commitment to the fibroblast lineage. In addition, microsatellite analysis was performed and documented for unambiguous parentage verification of clones in the future. Subsequently, the cryopreserved cells were tested for their suitability as nuclear donors (n = 7) using handmade cloning, and the reconstructed embryos were cultured in vitro. The cleavage rates (95.99% ± 2.17% vs. 82.18% ± 2.50%) and blastocyst rates (37.73% ± 1.54% vs. 24.31% ± ...Continue Reading

References

Oct 16, 1999·Reviews of Reproduction·W V Holt, A R Pickard
Apr 8, 2010·Journal of Photochemistry and Photobiology. B, Biology·André Passaglia Schuch, Carlos Frederico Martins Menck
Jan 1, 2008·Animal : an International Journal of Animal Bioscience·E GroeneveldNguyen Thi Vien
Jun 9, 2015·Cellular Reprogramming·Pankaj K MadheshiyaPrabhat Palta
Jul 29, 2016·PLoS Biology·Linn F GroeneveldPeer Berg

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Citations

Jan 7, 2020·Environmental Science and Pollution Research International·Hai-Ying ZhengJiang-Hua Shang
Feb 16, 2021·World Journal of Stem Cells·Dharmendra KumarWilfried A Kues

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Methods Mentioned

BETA
biopsies
biopsy
genotyping

Software Mentioned

Adobe Photoshop CS
STAT
SAS
BuNRCell
BuSwCell

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