Establishment of a Virulent Full-Length cDNA Clone for Type I Feline Coronavirus Strain C3663

Journal of Virology
Yutaka TeradaWataru Kamitani

Abstract

Feline infectious peritonitis (FIP) is one of the most important infectious diseases in cats and is caused by feline coronavirus (FCoV). Tissue culture-adapted type I FCoV shows reduced FIP induction in experimental infections, which complicates the understanding of FIP pathogenesis caused by type I FCoV. We previously found that the type I FCoV strain C3663 efficiently induces FIP in specific-pathogen-free cats through the naturally infectious route. In this study, we employed a bacterial artificial chromosome-based reverse genetics system to gain more insights into FIP caused by the C3633 strain. We successfully generated recombinant virus (rC3663) from Fcwf-4 cells transfected with infectious cDNA that showed growth kinetics similar to those shown by the parental virus. Next, we constructed a reporter C3663 virus carrying the nanoluciferase (Nluc) gene to measure viral replication with high sensitivity. The inhibitory effects of different compounds against rC3663-Nluc could be measured within 24 h postinfection. Furthermore, we found that A72 cells derived from canine fibroblasts permitted FCoV replication without apparent cytopathic effects. Thus, our reporter virus is useful for uncovering the infectivity of type I FCoV in...Continue Reading

References

Mar 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M L WellmanG J Kociba
Jul 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·C R GaushT F Smith
Sep 1, 1983·The Journal of General Virology·H E Jacobse-Geels, M C Horzinek
Aug 1, 1980·International Journal of Cancer. Journal International Du Cancer·S TsuchiyaK Tada
Apr 1, 1997·The Journal of Veterinary Medical Science·M MochizukiT Hohdatsu
Jun 28, 2002·The New England Journal of Medicine·Sharon WalmsleyUNKNOWN M98-863 Study Team
May 22, 2003·Journal of Virological Methods·Annamaria PratelliCanio Buonavoglia
Apr 9, 2004·Journal of Clinical Microbiology·Annamaria PratelliCanio Buonavoglia
Oct 1, 2005·Science·Wendong LiLin-Fa Wang
Oct 8, 2005·Clinical and Diagnostic Laboratory Immunology·Maya KummrowHans Lutz
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Susan R Weiss, Sonia Navas-Martin
Aug 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Wataru KamitaniShinji Makino
Nov 10, 2006·Journal of Wildlife Diseases·Roman BiekMary Poss
Jun 5, 2007·Veterinary Microbiology·Nozomi ShibaHiroyuki Iwata
Dec 14, 2007·Journal of Virology·Gergely TekesHeinz-Jürgen Thiel
Jun 14, 2008·PLoS Pathogens·Monique H VerheijeCornelis A M de Haan
Jul 15, 2011·The Journal of General Virology·Adriaan H de WildeMartijn J van Hemert
Aug 1, 2010·Viruses·Patrick C Y WooKwok-Yung Yuen
Jun 20, 2012·Emerging Infectious Diseases·Hui-Wen ChangPeter J M Rottier
Mar 23, 2013·The Journal of Veterinary Medical Science·Hiroshi ShimodaKen Maeda

❮ Previous
Next ❯

Citations

Sep 27, 2020·Journal of Virological Methods·Morgan PearsonGregg A Dean
Apr 4, 2021·Viruses·Makoto Ujike, Fumihiro Taguchi
Apr 14, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sailen Barik
Jan 1, 2020·Uirusu·Wataru Kamitani

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
reverse transcription-PCR
confocal microscopy
transfection
PCR
enzyme-linked immunosorbent assays

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.