Establishment of adoptive cell therapy with tumor infiltrating lymphocytes for non-small cell lung cancer patients

Cancer Immunology, Immunotherapy : CII
Ronny Ben-AviMichal J Besser

Abstract

Adoptive cell therapy (ACT) of tumor infiltration lymphocytes (TIL) yields promising clinical results in metastatic melanoma patients, who failed standard treatments. Due to the fact that metastatic lung cancer has proven to be susceptible to immunotherapy and possesses a high mutation burden, which makes it responsive to T cell attack, we explored the feasibility of TIL ACT in non-small cell lung cancer (NSCLC) patients. Multiple TIL cultures were isolated from tumor specimens of five NSCLC patients undergoing thoracic surgery. We were able to successfully establish TIL cultures by various methods from all patients within an average of 14 days. Fifteen lung TIL cultures were further expanded to treatment levels under good manufacturing practice conditions and functionally and phenotypically characterized. Lung TIL expanded equally well as 103 melanoma TIL obtained from melanoma patients previously treated at our center, and had a similar phenotype regarding PD1, CD28, and 4-1BB expressions, but contained a higher percent of CD4 T cells. Lung carcinoma cell lines were established from three patients of which two possessed TIL cultures with specific in vitro anti-tumor reactivity. Here, we report the successful pre-clinical prod...Continue Reading

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Citations

Mar 1, 2020·International Journal of Molecular Sciences·Linda Tran, Dan Theodorescu
Nov 25, 2018·Virchows Archiv : an International Journal of Pathology·Maartje W RohaanJohn B A G Haanen
Sep 2, 2020·International Journal of Molecular Sciences·Rajeev DhuparAdam C Soloff
Sep 18, 2020·Cells·Lena GaissmaierPetros Christopoulos
Mar 13, 2019·Journal of Experimental & Clinical Cancer Research : CR·Linan FangLi Zhang
May 31, 2020·Cancer Immunology, Immunotherapy : CII·Morten NielsenNiels Junker
Sep 15, 2018·Therapeutic Advances in Respiratory Disease·Nicolas Villanueva, Lyudmila Bazhenova
Feb 22, 2021·Japanese Journal of Clinical Oncology·Eiji NakataToshifumi Ozaki
Jun 19, 2021·Frontiers in Immunology·Amrendra KumarAnna E Vilgelm
Aug 31, 2021·Journal of Immunology Research·Xiaoyun LiYuan Yang
Oct 6, 2021·Journal for Immunotherapy of Cancer·Anders Handrup KvernelandInge Marie Svane

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