Establishment of an African green monkey model for COVID-19

BioRxiv : the Preprint Server for Biology
C. B. WoolseyRobert W Cross

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for an unprecedented global pandemic of COVID-19. Animal models are urgently needed to study the pathogenesis of COVID-19 and to screen candidate vaccines and treatments. Nonhuman primates (NHP) are considered the gold standard model for many infectious pathogens as they usually best reflect the human condition. Here, we show that African green monkeys support a high level of SARS-CoV-2 replication and develop pronounced respiratory disease that may be more substantial than reported for other NHP species including cynomolgus and rhesus macaques. In addition, SARS-CoV-2 was detected in mucosal samples of all animals including feces of several animals as late as 15 days after virus exposure. Importantly, we show that virus replication and respiratory disease can be produced in African green monkeys using a much lower and more natural dose of SARS-CoV-2 than has been employed in other NHP studies.

Citations

Oct 1, 2020·Scientific Reports·Yuan ZhaoXiaozhong Peng
Jul 2, 2020·Biology·Saurabh PandeyDeeksha Tripathi
Jun 28, 2020·Journal of Virology·John P Moore, P J Klasse
Oct 8, 2020·Critical Care : the Official Journal of the Critical Care Forum·Salleh N EhaidebAbderrezak Bouchama
Dec 2, 2020·Nature Communications·Fatai S OladunniJordi B Torrelles
Oct 30, 2020·Nature Reviews. Microbiology·Philip V'kovskiVolker Thiel
Nov 4, 2020·Nature Reviews. Immunology·David S KhouryMiles P Davenport
Nov 12, 2020·Science·Bas B Oude MunninkMarion P G Koopmans
Sep 6, 2020·Disease Models & Mechanisms·Sarah R LeistDavid R Martinez
Apr 25, 2021·The Veterinary Quarterly·Khan SharunYoussef A Attia
Jul 17, 2021·Scientific Reports·Magen E FrancisAlyson A Kelvin

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Methods Mentioned

BETA
bronchoalveolar
lavage
ELISA
sedation
PCR
flow cytometry
FACS

Software Mentioned

BioLegend
Maven Patient Image
CFX Manager
BD FACS Diva

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