Establishment of an antiviral assay system and identification of severe fever with thrombocytopenia syndrome virus inhibitors

Antiviral Chemistry & Chemotherapy
Masanori BabaMasayuki Saijo

Abstract

Aims Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne infectious disease. SFTS is epidemic in Asia, and its fatality rate is around 30% in Japan. The causative virus severe fever with thrombocytopenia syndrome virus (SFTSV) is a phlebovirus of the family Phenuiviridae (the order Bunyavirales). Although effective treatments are required, there are no antiviral agents currently approved for clinical use. Ribavirin and favipiravir were examined for their anti-SFTSV activity and found to be selective inhibitors of SFTSV replication in vitro. However, their activity was not sufficient. Therefore, it is mandatory to identify novel compounds active against SFTSV. To this end, we have established a safe and rapid assay system for screening selective inhibitors of SFTSV. Methods The virus was isolated from SFTS patients treated in Kagoshima University Hospital. Vero cells were infected with SFTSV and incubated in the presence of various concentrations of test compounds. After three days, the cells were examined for their intracellular viral RNA levels by real-time reverse transcription-PCR without extracting viral RNA. The cytotoxicity of test compounds was determined by a tetrazolium dye method. Results Amon...Continue Reading

References

Dec 21, 2007·Antiviral Research·Colleen B JonssonGregory Mertz
Mar 18, 2011·The New England Journal of Medicine·Xue-Jie YuDe-Xin Li
Apr 13, 2011·The Journal of Antimicrobial Chemotherapy·Sibel AsciogluK Arnold Chan
Nov 17, 2011·Biochemical and Biophysical Research Communications·Mohammed T A SalimMasanori Baba
Aug 7, 2013·Antimicrobial Agents and Chemotherapy·Hidehiro SangawaYousuke Furuta
Nov 12, 2013·Emerging Infectious Diseases·Kye-Hyung KimMyoung-don Oh
Apr 1, 2014·Antiviral Research·Siwaporn BoonyasuppayakornRadhakrishnan Padmanabhan
Nov 21, 2014·Japanese Journal of Infectious Diseases·Masayuki ShimojimaMasayuki Saijo
Feb 20, 2015·Emerging Infectious Diseases·Tomoya SaitoKensuke Nakajima
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Jun 10, 2016·Clinical Microbiology Reviews·Erik De Clercq, Guangdi Li
Aug 10, 2016·Antiviral Chemistry & Chemotherapy·Wataru ItoMasanori Baba

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