Establishment of an ELISA system for determining soluble LAIR-1 levels in sera of patients with HFRS and kidney transplant

Journal of Immunological Methods
Weiming OuyangBoquan Jin

Abstract

LAIR-1, the leukocyte-associated Ig-like receptor-1, is a trans-membrane molecule that functions as an inhibitory receptor on natural killer cells, T lymphocytes and monocytes. It has been well known that many trans-membrane receptors can shed from the cell surface and be released into the circulation in soluble form when lymphocytes, endothelials and other immune cells are activated. In many cases, the levels of soluble receptors in the circulation can be used as markers of lymphocyte activation in transplant patients and virus infection patients. To investigate whether LAIR-1 is able to be released into the sera, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) system based on two anti-LAIR-1 monoclonal antibodies (MAb) with different epitope specificities. Using this ELISA, we found that sLAIR-1 existed in the supernatants collected from PMA, PHA or CD3 MAb-stimulated lymphocytes cultures in vitro for the first time. Moreover, we found that LAIR-1 level in serum samples from healthy individuals was 6.2 +/- 3.3 ng/ml, whereas the levels in sera of patients with hemorrhagic fever with renal syndrome (HFRS) and patients 3-7 days after kidney transplant increased to 47.2 +/- 35.9 and 24.4 +/- 16.0 ng/ml, respect...Continue Reading

References

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Oct 16, 2003·Biochemical and Biophysical Research Communications·Weiming OuyangBoquan Jin

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Citations

Oct 2, 2012·Indian Journal of Clinical Biochemistry : IJCB·Simin SohrabiSoheil Ghassemi
May 22, 2013·PloS One·Rita SimoneDaniele Saverino
Apr 4, 2014·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Xin XieBoquan Jin
Aug 21, 2013·Journal of Immunological Methods·Xin XieBoQuan Jin
Dec 26, 2006·Molecular Immunology·Robert Jan Lebbink, Linde Meyaard
Sep 2, 2008·Immunological Reviews·Alexander David Barrow, John Trowsdale
Nov 28, 2012·The Urologic Clinics of North America·Herman Singh BaggaMarshall L Stoller
Apr 1, 2017·BMC Bioinformatics·Wei-Sheng TienKun-Pin Wu
May 11, 2010·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Ying MaBoquan Jin
Sep 29, 2017·Current Allergy and Asthma Reports·Anna S Tocheva, Adam Mor
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert Jan LebbinkLinde Meyaard
Apr 26, 2019·Journal of Immunology Research·María Martínez-EsparzaPilar García-Peñarrubia
Dec 3, 2020·Frontiers in Immunology·Claire E McQuittyLuca Urbani

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