Establishment of an immortalized GABAergic neuronal progenitor cell line from embryonic ventral mesencephalon in the rat

Brain Research
Beibei WangQunyuan Xu

Abstract

Effective cell replacement therapies for neurological disease require neuron-restricted precursors as grafted cells. The problem of obtaining sufficient grafts for transplantation can be resolved by creating an appropriate immortalized cell line. In the present study, a thermally controlled immortalized GABAergic neuronal progenitor cell line (RMNE6) was established from E13 rat ventral mesencephalon cells immortalized using the temperature-sensitive mutant of SV40 large T antigen (ts-TAg). RMNE6 cells proliferated rapidly and expressed a neuron-like phenotype at the permissive temperature (33 degrees C), but eventually stopped growing at the non-permissive temperature (39 degrees C). Expression of the neuronal markers PSA-NCAM, beta-tubulin III and MAP2 by RMNE6 cells was confirmed by RT-PCR or immunocytochemistry. Furthermore, these cells exhibited functional GABAergic neuron properties, as evidenced by the expression of glutamate decarboxylase (GAD) as well as the synthesis and release of the neurotransmitter GABA in a calcium-dependent manner. Moreover, RMNE6 cells spontaneously expressed and secreted several neurotrophic factors, such as NGF, BDNF, NT-3, NT-4/5, and GDNF. The cells survived well and kept expression of SV40...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E M EvesB H Wainer
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C EvrardP Rouget
Aug 1, 1988·Neuron·K FrederiksenR McKay
Sep 1, 1983·Nature·F Bourre, A Sarasin
Sep 1, 1994·Journal of Neuroscience Research·P S FrisaJ W Jacobberger
Nov 1, 1994·The European Journal of Neuroscience·H R Widmer, F Hefti
Sep 24, 1993·Annals of the New York Academy of Sciences·E E Baetge
Jul 22, 1998·Journal of Neuroscience Research·E Cattaneo, L Conti
May 29, 2000·Brain Research·I FerrerT Ribalta
Jul 7, 2000·Molecular and Cellular Neurosciences·F J RubioA Martínez-Serrano
Feb 13, 2001·Experimental Neurology·M E EhrlichE Cattaneo
Sep 7, 2001·Cytokine & Growth Factor Reviews·M Takahashi
May 4, 2002·Nature Reviews. Neuroscience·Ferdinando Rossi, Elena Cattaneo
Jul 3, 2002·Nature Reviews. Neuroscience·Nicolas BertrandFrançois Guillemot
Mar 3, 2004·Journal of Neural Transmission·A SalehiD F Swaab
May 4, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Adrian P KellsBronwen Connor
Jan 13, 2006·Cell and Tissue Research·Joseph F SanchezWilliam J Freed
Mar 11, 2006·Neuron Glia Biology·Sarina B ElmariahRita J Balice-Gordon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.