Establishment of an optimal hypo-osmotic swelling test by examining single spermatozoa in four different hypo-osmotic solutions

Human Reproduction
Y L TsaiT A Baramki

Abstract

In order to find an optimal hypo-osmotic swelling test (HOST) and to identify viable sperm cells from patients with asthenozoospermia for intracytoplasmic sperm injection (ICSI), we tested single motile and non-motile spermatozoa in four hypo-osmotic solutions by micromanipulation. The four solutions were: A, H2O; B, 50 mOsm NaCl; C, 150 mOsm NaCl and D, 150 mOsm sodium citrate and fructose. Eosin Y staining was then carried out for evaluation of viability of the spermatozoa after HOST. Using motile spermatozoa, no statistical difference was found in HOST-positive spermatozoa between these four solutions. There were more viable sperm cells after HOST in solutions C and D, as noted by Eosin Y staining. After non-motile spermatozoa were incubated for 1 min in the four solutions, HOST with solution C gave the best results for identification of viable sperm cells compared to the other three solutions. When motile spermatozoa were incubated in solution C or solution D for 30 min, the result of HOST with solution C (10.8% dead spermatozoa) was superior to that of solution D (49.1% dead spermatozoa). In conclusion, the HOST protocol using 150 mOsm NaCl (solution C) for 1 min yielded the best results for selection of viable spermatozoa...Continue Reading

Citations

Aug 15, 2006·The Journal of Reproduction and Development·Taiki MatsuokaYutaka Fukui
Sep 14, 2010·Reproductive Biomedicine Online·James D StangerGhanim Almahbobi
Aug 9, 2008·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Janos KoncSandor Cseh
Mar 24, 2017·Basic and Clinical Andrology·Greta VerheyenHerman Tournaye
Jul 3, 2021·Journal of Clinical Medicine·Kaan Aydos, Oya Sena Aydos

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