Establishment of an outgrowth culture system to study growth regulation of normal human epithelium

In Vitro Cellular & Developmental Biology. Animal
T Masui

Abstract

To study the growth regulation of epithelial cells as a sheet, I developed an outgrowth culture system for normal human ectocervical epithelial (NHCE) cells, whereby outgrowths from tissue explants increase their radius in a constant rate over time. Cinematographic observation revealed that throughout the outgrowths the cells coordinately migrate and proliferate. To date, all 59 specimens examined have shown similar growth characteristics, with explant size not causing any difference in the growth rate; 10(8) cells/specimen can easily be obtained in 3 wk. Cell densities of outgrowths also remain constant. Moreover, there is no fibroblast contamination, and removal of explants does not affect growth rate. Therefore, pure epithelial outgrowth in uniform growth condition can be prepared for further experiments. The results demonstrate that the outgrowth culture system is an attractive model for analysis of growth control mechanisms in normal human epithelium in vitro.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R W HolleyY C Yeh
Oct 15, 1979·International Journal of Cancer. Journal International Du Cancer·M A Stanley, E K Parkinson
Jul 6, 1978·Nature·A S Curtis, G M Seehar
Oct 1, 1992·Journal of Cellular Physiology·M S PepperJ D Vassalli
Mar 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·B L Coomber, A I Gotlieb
Jun 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M E TurykG D Wilbanks
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T MasuiC C Harris
Jan 1, 1980·Methods in Cell Biology·G D StonerC C Harris
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M VerderameR Pollack
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S L HammondM R Stampfer
Jan 1, 1984·Differentiation; Research in Biological Diversity·J F LechnerA M Shamsuddin
Apr 1, 1993·The Journal of Cell Biology·R G SosnowskiJ R Feramisco

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.