Establishment of biological and pharmacokinetic assays of telomerase-specific replication-selective adenovirus

Cancer Science
Yuuri HashimotoToshiyoshi Fujiwara

Abstract

The use of replication-selective tumor-specific viruses represents a novel approach for the treatment of neoplastic disease. We constructed an attenuated adenovirus, telomerase-specific replication-selective adenovirus (TRAD), in which the human telomerase reverse transcriptase promoter element drives the expression of the E1A and E1B genes linked with an internal ribosome entry site (IRES). Forty-eight hours after TRAD infection at a multiplicity of infection of 1.0, the cell viability of H1299 human lung cancer cells was consistently less than 50% and therefore this procedure could be used as a potency assay to assess the biological activity of TRAD. We also established a quantitative real-time polymerase chain reaction (PCR) analysis with consensus primers for either the adenovirus E1A or IRES sequence. The linear ranges of quantitation with E1A and IRES primers were 10(3)-10(8) and 10(2)-10(8) plaque-forming units/mL in the plasma, respectively. The PCR analysis demonstrated that the levels of E1A in normal tissues were more than 10(3) lower than in the tumors of A549 human lung tumor xenografts in nu/nmicro mice at 28 days after intratumoral injection. Our results suggest that the cell-killing assay against H1299 cells and...Continue Reading

References

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Citations

Jan 6, 2009·Uirusu·Toshiyoshi Fujiwara, Noriaki Tanaka
Jan 5, 2011·Journal of Cancer Research and Clinical Oncology·Guidong LiNaoto Endo
Apr 21, 2011·Expert Review of Anticancer Therapy·Toshiyoshi FujiwaraShunsuke Kagawa
Oct 11, 2013·Expert Opinion on Biological Therapy·Hiroshi TazawaToshiyoshi Fujiwara
Feb 17, 2009·Expert Opinion on Biological Therapy·Toshiyoshi Fujiwara
Nov 20, 2012·International Journal of Cancer. Journal International Du Cancer·Toshiaki OharaToshiyoshi Fujiwara
Apr 12, 2012·International Journal of Cancer. Journal International Du Cancer·Hiroshi TazawaToshiyoshi Fujiwara
Jan 17, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Yasumoto YamasakiToshiyoshi Fujiwara
Dec 20, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Satoru KikuchiToshiyoshi Fujiwara
Dec 14, 2018·Oncoimmunology·Jonathan G PolLorenzo Galluzzi
Jul 28, 2019·Annals of Gastroenterological Surgery·Toshiyoshi Fujiwara
Nov 6, 2020·Cancer Immunology, Immunotherapy : CII·Yusuke MochizukiToshiyoshi Fujiwara
Mar 26, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yuji KuriharaToshiyoshi Fujiwara
Feb 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tsuyoshi SasakiToshiyoshi Fujiwara
Oct 2, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shuya YanoToshiyoshi Fujiwara
Dec 3, 2015·Molecular Cancer Therapeutics·Kiyoto TakeharaToshiyoshi Fujiwara
Mar 20, 2021·Journal of Medical Virology·Weijian LinLiping Zhong
Jun 12, 2021·Cancer Chemotherapy and Pharmacology·Kazuhisa SugiuToshiyoshi Fujiwara
Jun 22, 2021·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Yasuhiro ShirakawaToshiyoshi Fujiwara
May 24, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yoshihiko KakiuchiToshiyoshi Fujiwara

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