PMID: 11336358May 5, 2001Paper

Establishment of bone marrow-derived endothelial cell lines from ts-SV40 T-antigen gene transgenic rats

Pharmaceutical Research
K HattoriE Nakashima

Abstract

Postneonatal neovascularization is thought to result exclusively from the proliferation, migration, and remodeling of fully differentiated endothelial cells (ECs). Recently, it has been reported that bone marrow contains cells which can differentiate into ECs and contribute to neoangiogenesis in adult species. In this study, we tried to establish conditionally immortalized endothelial cell lines (TR-BME) derived from rat bone marrow. Mononuclear cells were isolated and differentiated into ECs at 37 degrees C from the bone marrow of a transgenic rat harboring temperature-sensitive SV40 large T-antigen (ts T-Ag) gene. Then, the cells were transferred and incubated at 33 degrees C, a permissive temperature for ts T-Ag. Expression of vascular endothelial growth factor (VEGF) receptor (VEGFR)-1, 2, Tie-1, 2 and von Willebrand factor (VWF) were assayed by reverse transcriptase-mediated polymerase chain reaction (RT-PCR). We have established three cell lines incorporating 1,1'-dioctadecyl-3,3,3',3-tetramethylindo-carbocyanine perchlorate (DiI-Ac-LDL) with a spindle shape. One of these, clone 2, strongly expressed VEGFR-2, and weakly expressed VEGFR-1 and VWF. In contrast, clone 8 showed strong expression of Tie-1, 2, and VWF, and weak...Continue Reading

Citations

Jan 19, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Maria Luisa Brandi, Patricia Collin-Osdoby
Jun 10, 2004·Biological & Pharmaceutical Bulletin·Tomohide KitanoEmi Nakashima
Jan 7, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mariko MutaMasakazu Toi
Oct 14, 2003·Drug Discovery Today·Tetsuya TerasakiKen-ichi Hosoya

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