Establishment of cell-based transposon-mediated transgenesis in cattle

Theriogenology
Ana P AlessioPablo Bosch

Abstract

Transposon-mediated transgenesis is a well-established tool for genome modification in small animal models. However, translation of this active transgenic method to large animals warrants further investigations. Here, the piggyBac (PB) and sleeping beauty (SB) transposon systems were assessed for stable gene transfer into the cattle genome. Bovine fibroblasts were transfected either with a helper-independent PB system or a binary SB system. Both transposons were highly active in bovine cells increasing the efficiency of DNA integration up to 88 times over basal nonfacilitated integrations in a colony formation assay. SB transposase catalyzed multiplex transgene integrations in fibroblast cells transfected with the helper vector and two donor vectors carrying different transgenes (fluorophore and neomycin resistance). Stably transfected fibroblasts were used for SCNT and on in vitro embryo culture, morphologically normal blastocysts that expressed the fluorophore were obtained with both transposon systems. The data indicate that transposition is a feasible approach for genetic engineering in the cattle genome.

References

Oct 21, 1994·Cell·H G van LuenenR H Plasterk
Aug 4, 1999·Trends in Genetics : TIG·R H PlasterkZ Ivics
Feb 5, 2000·Molecular Reproduction and Development·G VajtaH Callesen
Jul 5, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Aron M GeurtsPerry B Hackett
Oct 8, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jacob Giehm MikkelsenMark A Kay
Mar 4, 2005·Molecular and Cellular Biology·Stephen R YantMark A Kay
Jan 4, 2006·Journal of Biotechnology·Changsoo LeeSeokhwan Hwang
Dec 14, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Matthew H WilsonAlfred L George
Dec 21, 2006·Molecular and Cellular Biology·Ryuji IkedaJunji Takeda
Jan 2, 2007·Nature Biotechnology·Jürgen A RichtYoshimi Kuroiwa
Jul 21, 2007·BMC Biotechnology·Karl J ClarkScott C Fahrenkrug
Mar 21, 2008·The EMBO Journal·Rupak MitraNancy L Craig
Sep 27, 2008·Science·Christopher S RogersMichael J Welsh
Jul 9, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xia WangJayanta Roy-Chowdhury
Apr 8, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ivana GrabundzijaZoltán Ivics
Apr 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Johann UrschitzStefan Moisyadi
Jul 16, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jason B BellPerry B Hackett
Jul 31, 2010·Nature Protocols·Anna ParuzynskiChristof von Kalle
Aug 31, 2010·Transgenic Research·Jannik E JakobsenJacob Giehm Mikkelsen
Jan 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kosuke YusaNancy L Craig
Jan 12, 2011·Transgenic Research·Daniel F CarlsonScott C Fahrenkrug
Jan 1, 2007·CSH Protocols·Chuan-Wei Jang, Richard R Behringer
Jun 24, 2011·Biotechnology Letters·Shengwei HuChuangfu Chen
Jul 13, 2011·The Journal of Veterinary Medical Science·Su KimGoo Jang
Oct 1, 2011·Nature Protocols·Zoltán IvicsF Kent Hamra
Apr 12, 2012·Nucleic Acids Research·Jesse B OwensStefan Moisyadi
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tae Sub Park, Jae Yong Han
Jun 29, 2012·Bioscience, Biotechnology, and Biochemistry·Ding-Ping BaiYu-Lin Chen
Oct 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Joel MarhStefan Moisyadi
Dec 1, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katharina KatterZsuzsanna Izsvák
Mar 15, 2014·Nature Protocols·Zoltán IvicsZsuzsanna Izsvák
Mar 15, 2014·Nature Protocols·Zoltán IvicsZsuzsanna Izsvák
Mar 15, 2014·Nature Protocols·Zoltán IvicsZsuzsanna Izsvák

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Citations

Oct 5, 2016·Critical Reviews in Biochemistry and Molecular Biology·Suneel A NarayanavariZsuzsanna Izsvák
Feb 10, 2018·Journal of Animal Science and Biotechnology·Soo-Young YumGoo Jang

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