PMID: 9557974Apr 29, 1998Paper

Establishment of conditionally immortalized rat utricular epithelial cell lines using a retrovirus-mediated gene transfer technique

Hearing Research
J L ZhengW Q Gao

Abstract

Supporting cells in the inner ear sensory epithelium are most likely hair cell progenitors. In an effort to establish an in vitro model system of hair cell differentiation, we developed immortalized epithelial cell lines by transferring the tsA58 allele of the SV40 large T antigen oncogene into neonatal rat utricular supporting cells using a retrovirus. The established cell lines have been stably maintained continuously for more than 25 passages and display many features similar to primary supporting cells. They grow in patches and assume a polygonal morphology. Immunocytochemical characterization of the established cell lines reveals that these cells can be labeled by epithelial cell markers, but not by fibroblast, glial or neuronal markers. The immortalized cells grow rapidly in serum medium at permissive temperature, but the majority cease proliferation when cultured in serum free medium at non-permissive temperature. These cells respond to mitogenic growth factors including bFGF, EGF and TGF-alpha and express growth factor receptors in a manner similar to the primary supporting cells. Furthermore, we find that the cells undergo a morphological differentiation when cultured in serum free medium at non-permissive temperature ...Continue Reading

References

Apr 1, 1990·Trends in Neurosciences·U Lendahl, R D McKay
Jan 1, 1992·Experimental Neurology·P Weisleder, E W Rubel
Jun 1, 1990·The Journal of Cell Biology·E DiCicco-BloomI B Black
Jun 24, 1988·Science·J T Corwin, D A Cotanche
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·H Yamashita, E C Oesterle
Aug 22, 1994·The Journal of Comparative Neurology·C J DechesneG Desmadryl
Jan 1, 1995·Acta Oto-laryngologica. Supplementum·H YamaneY Nakai
Jul 1, 1997·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·K F BaraldA Poniatowski
Jul 1, 1997·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M C HolleyN Grix
Jan 1, 1997·Audiology & Neuro-otology·M C Holley, P W Lawlor

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Citations

May 29, 2008·Journal of the Association for Research in Otolaryngology : JARO·Anping XiaJohn S Oghalai
Jun 11, 2011·Journal of Molecular Histology·Robert J Marano, Sharon L Redmond
Sep 30, 1998·Current Opinion in Neurobiology·H Staecker, T R Van De Water
Mar 10, 2011·Journal of Speech, Language, and Hearing Research : JSLHR·Mark A Parker
May 2, 2001·Journal of Neurochemistry·E CarniceroT Schimmang
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·J L Cyr, A J Hudspeth
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·J S Stone, E W Rubel
Oct 1, 1998·Proceedings. Biological Sciences·M N RivoltaM C Holley
Aug 31, 2004·Journal of Neuroscience Research·Estela CarniceroThomas Schimmang
Oct 23, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·G Lawoko-KeraliM C Holley
Mar 9, 2004·Mechanisms of Development·Grace Lawoko-KeraliMatthew C Holley
Jun 1, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ida M StoneDavid J Poulsen
Jul 25, 2000·The Journal of Comparative Neurology·E C OesterleM D Coltrera
Sep 13, 2005·Journal of Neurobiology·Suoqiang ZhaiWei-Qiang Gao
Oct 17, 2002·Journal of Neurobiology·Marcelo N Rivolta, Matthew C Holley

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